Molecular recognition of an acyl-enzyme intermediate on the lipase B from Candida antarctica

Articulo

Autoría:

TOLEDO, MARIA VICTORIA ; LLERENA SUSTER, CARLOS RAFAEL ; FERREIRA, MARIA LUJAN ; COLLINS, SEBASTIAN ENRIQUE ; BRIAND, LAURA ESTEFANIA

Fecha:

2017

Editorial y Lugar de Edición:

Royal Society of Chemistry

Revista:

Catalysis Science and technology, vol. 7 (pp. 1953-1964) - ISSN 2044-4761
Royal Society of Chemistry

ISSN:

2044-4761

Resumen

This investigation provides evidences of the acyl enzyme species involved in theinteraction of R/S-ketoprofen with the lipase B from Candida antarctica. The interaction between the profen and the enzyme was studied by in situ Time-Resolved ATR-FTIR both in static and transient conditions. Particularly, modulation excitation spectroscopy (MES) with phase sensitive detection (PSD) allowed to univocally distinguishing the signals belonging to the interaction between ketoprofen and the enzyme from the strong background signals. These experimental tools coupled with theoretical DFT analysis allowed to propose various species due to the interaction of ketoprofen with serine through H bonding (without reaction) and the acyl enzyme species (ester bond formation) that are the intermediates in the biocatalytic assisted esterification andhydrolysis using lipases.

Palabras Clave

R/S-ketoprofeninfrared spectroscopylipaseacyl-enzyme

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http://hdl.handle.net/11336/31389