Nutraceutico. Hipoxia. Proteinas de membrana. Especies Reactivas del oxigen y Nitrogeno
Disciplina Científica
Bioquímica y Biología Molecular - Biología
Tema
Fideos nutracéuticos a base de complejos tipo V de almidones modificados/polifenoles obtenidos in situ por extrusión
Lugar de Trabajo
DEPARTAMENTO DE QUIMICA
Depende de
UNIVERSIDAD DE BELGRANO
FACULTAD DE CS.EXACTAS Y NATURALES
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Dirección:
VILLANUEVA 1324, 1426 - Capital Federal - Argentina
ResumenInformación suministrada por el agente en SIGEVA
Cell Culture: Cell culture experience with yeast and bacteria. Expert fermentor operator.Cell culture experience with mammalian cell lines and primary cell cultures, transient and stable transfections, adenoviral and retroviral infections. DNA, RNA: RNA extraction, RNA quantification (Q-PCR), Northern blots. RNA and DNA manipulation. Molecular cloning, minipreps, maxipreps, gel shiftsMitochondria: More than 8 years of working with mitochondria isolation from several sources includind tissues an...Cell Culture: Cell culture experience with yeast and bacteria. Expert fermentor operator.Cell culture experience with mammalian cell lines and primary cell cultures, transient and stable transfections, adenoviral and retroviral infections. DNA, RNA: RNA extraction, RNA quantification (Q-PCR), Northern blots. RNA and DNA manipulation. Molecular cloning, minipreps, maxipreps, gel shiftsMitochondria: More than 8 years of working with mitochondria isolation from several sources includind tissues and cells., Mitochondria bioenergetics, respiration and oxidative phosphorylation analysis, production of free radicals, measurement of membrane potential, mitochondria engineering, protein analysis, by several methods, enzyme analysis, measurement of cytochromes interaction with xenobiotics, isolation of mitochondria and subfractionation of mitochondriaProteins: Strong background in cloning, expression and protein purification from natural and recombinant sources using several techniques: differential centrifugation, chromatography (FPLC, ion-exchange, affinity, size exclusion, HPLC).
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Líneas de Investigación
Modulacion de radicales libres por factores ambientales