1. CONICET
  2. CONICET Community
  3. PABLO RAUL CASTELLO
CONICET Community
CASTELLO, PABLO RAUL

Independent researcher

Speciality
Nutraceutico. Hipoxia. Proteinas de membrana. Especies Reactivas del oxigen y Nitrogeno
Scientific discipline
Biochemistry and Molecular Biology - Biology
Topic
Nutraceutical noodles based on type V complexes of modified starches/polyphenols obtained in situ by extrusion
Workplace
DEPARTAMENTO DE QUIMICA
Dependencies
  • UNIVERSIDAD DE BELGRANO
    • FACULTAD DE CS.EXACTAS Y NATURALES
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Address:
VILLANUEVA 1324, 1426 - Capital Federal - Argentina
Summary Information provided by the agent in SIGEVA
Cell Culture: Cell culture experience with yeast and bacteria. Expert fermentor operator.Cell culture experience with mammalian cell lines and primary cell cultures, transient and stable transfections, adenoviral and retroviral infections. DNA, RNA: RNA extraction, RNA quantification (Q-PCR), Northern blots. RNA and DNA manipulation. Molecular cloning, minipreps, maxipreps, gel shiftsMitochondria: More than 8 years of working with mitochondria isolation from several sources includind tissues an... Cell Culture: Cell culture experience with yeast and bacteria. Expert fermentor operator.Cell culture experience with mammalian cell lines and primary cell cultures, transient and stable transfections, adenoviral and retroviral infections. DNA, RNA: RNA extraction, RNA quantification (Q-PCR), Northern blots. RNA and DNA manipulation. Molecular cloning, minipreps, maxipreps, gel shiftsMitochondria: More than 8 years of working with mitochondria isolation from several sources includind tissues and cells., Mitochondria bioenergetics, respiration and oxidative phosphorylation analysis, production of free radicals, measurement of membrane potential, mitochondria engineering, protein analysis, by several methods, enzyme analysis, measurement of cytochromes interaction with xenobiotics, isolation of mitochondria and subfractionation of mitochondriaProteins: Strong background in cloning, expression and protein purification from natural and recombinant sources using several techniques: differential centrifugation, chromatography (FPLC, ion-exchange, affinity, size exclusion, HPLC).
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Lines of Investigation

Modulacion de radicales libres por factores ambientales

Natural and exact sciences

  • Biological sciences
  • Biophysics
Technological Capacities

5 - Physical and exact sciences

5.1 - Chemistry

  • 5.1.1 - Analytical Chemistry
  • 5.1.3 - Inorganic Chemistry
  • 5.1.4 - Organic Chemistry

5.4 - Separation Technologies

  • 5.4.1 - Filtration and Membrane Processes
  • 5.4.2 - Extraction
  • 5.4.3 - Adsorption
  • 5.4.4 - Distillation

6 - Biological sciences

6.2 - Biology/Biotechnology

  • 6.2.1 - Biochemistry/Biophysics
  • 6.2.2 - Cellular and Molecular Biology
  • 6.2.3 - Genetic Engineering
  • 6.2.4 - In vitro Testing, Trials
Key Words
MitocondriaHipoxiaRadicales libresFree ReadicalsCampos MagneticosMitochondriaHypoxiaMagnetic fields
Education

1992 - 1998

Doctor de la Universidad de Buenos Aires

UNIVERSIDAD DE BUENOS AIRES

1984 - 1994

Bioquimico

UNIVERSIDAD DE BUENOS AIRES

1984 - 1989

Farmaceutico

UNIVERSIDAD DE BUENOS AIRES

HR Training
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