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  3. PABLO RAUL CASTELLO
CONICET Community

CASTELLO, PABLO RAUL

Independent researcher

SPECIALITY:

Nutraceutico. Hipoxia. Proteinas de membrana. Especies Reactivas del oxigen y Nitrogeno

Scientific discipline:

Biochemistry and Molecular Biology - Biology

Topic:

Nutraceutical noodles based on type V complexes of modified starches/polyphenols obtained in situ by extrusion

Workplace

DEPARTAMENTO DE QUIMICA Depends on
  • UNIVERSIDAD DE BELGRANO (UB)
    • FACULTAD DE CS.EXACTAS Y NATURALES
Address:
VILLANUEVA 1324, 1426 - Capital Federal - Argentina

Contact:

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S&T Expertise*

Cell Culture: Cell culture experience with yeast and bacteria. Expert fermentor operator.Cell culture experience with mammalian cell lines and primary cell cultures, transient and stable transfections, adenoviral and retroviral infections. DNA, RNA: RNA extraction, RNA quantification (Q-PCR), Northern blots. RNA and DNA manipulation. Molecular cloning, minipreps, maxipreps, gel shiftsMitochondria: More than 8 years of working with mitochondria isolation from several sources includind tissues and cells., Mitochondria bioenergetics, respiration and oxidative phosphorylation analysis, production of free radicals, measurement of membrane potential, mitochondria engineering, protein analysis, by several methods, enzyme analysis, measurement of cytochromes interaction with xenobiotics, isolation of mitochondria and subfractionation of mitochondriaProteins: Strong background in cloning, expression and protein purification from natural and recombinant sources using several techniques: differential centrifugation, chromatography (FPLC, ion-exchange, affinity, size exclusion, HPLC). *Information provided by the agent in SIGEVA

Lines of Investigation

Modulacion de radicales libres por factores ambientales Natural and exact sciences - Biological sciences - Biophysics

Technological Capacities

  • 5. Physical and exact sciences
    • 1. Chemistry
      • 1.1. Analytical Chemistry
      • 1.3. Inorganic Chemistry
      • 1.4. Organic Chemistry
    • 4. Separation Technologies
      • 4.1. Filtration and Membrane Processes
      • 4.2. Extraction
      • 4.3. Adsorption
      • 4.4. Distillation
  • 6. Biological sciences
    • 2. Biology/Biotechnology
      • 2.1. Biochemistry/Biophysics
      • 2.2. Cellular and Molecular Biology
      • 2.3. Genetic Engineering
      • 2.4. In vitro Testing, Trials

Key Words

MitocondriaHipoxiaRadicales libresFree ReadicalsCampos MagneticosMitochondriaHypoxiaMagnetic fields

Education

1992 - 1998

Doctor de la Universidad de Buenos Aires

UNIVERSIDAD DE BUENOS AIRES

1984 - 1994

Bioquimico

UNIVERSIDAD DE BUENOS AIRES

1984 - 1989

Farmaceutico

UNIVERSIDAD DE BUENOS AIRES


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