Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography

Artículo

Autoría

Saavedra, Soledad Lorena ; MARTINEZ CERON, MARIA CAMILA ; GIUDICESSI, SILVANA LAURA ; Forno, Angela Guillermina ; Bosco, Maria Belen ; MARANI, MARIELA MIRTA ; ERRA BALSELLS, ROSA ; Albericio Palomera, Fernando ; Cascone, Osvaldo ; CAMPERI, SILVIA ANDREA

Fecha

2018

Editorial y Lugar de Edición

American Chemical Society

Revista

BIOTECHNOLOGY PROGRESS, vol. 34 (pp. 999-1005) American Chemical Society

Resumen Información suministrada por el agente en SIGEVA

Recombinant human growth hormone (rhGH) is used for the treatment of several pathologies, most of them related to growth. Although different expression systems can be used for its production, the milk from transgenic cows is one of the most interesting due to the high rhGH level achieved (5 g/L). We have designed and synthesized short peptides (9 or 10 amino acid long) using Fmoc chemistry and studied their ability to purify rhGH from milk once immobilized on an agarose support. Using spiked mi... Recombinant human growth hormone (rhGH) is used for the treatment of several pathologies, most of them related to growth. Although different expression systems can be used for its production, the milk from transgenic cows is one of the most interesting due to the high rhGH level achieved (5 g/L). We have designed and synthesized short peptides (9 or 10 amino acid long) using Fmoc chemistry and studied their ability to purify rhGH from milk once immobilized on an agarose support. Using spiked milk with the hormone as a sample, rhGH was purified with 88% yield and 92% purity in a single step with a fold purification of 4.5.

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Palabras Clave

DOWNSTREAM PROCESSINGTRANSGENIC COW MILKBIOLOGICSSOLID-PHASE PEPTIDE SYNTHESISRECOMBINANT BIOPHARMACEUTICALSPEPTIDE LIGANDS

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http://hdl.handle.net/11336/86759