Science and Technology Production
Article
Authorship
Saavedra, Soledad Lorena
;
MARTINEZ CERON, MARIA CAMILA
;
GIUDICESSI, SILVANA LAURA
;
Forno, Angela Guillermina
;
Bosco, Maria Belen
;
MARANI, MARIELA MIRTA
;
ERRA BALSELLS, ROSA
;
Albericio Palomera, Fernando
;
Cascone, Osvaldo
;
CAMPERI, SILVIA ANDREA
Date
2018
Publishing House and Editing Place
American Chemical Society
Magazine
BIOTECHNOLOGY PROGRESS,
vol. 34
(pp. 999-1005)
American Chemical Society
Summary
Information provided by the agent in
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Recombinant human growth hormone (rhGH) is used for the treatment of several pathologies, most of them related to growth. Although different expression systems can be used for its production, the milk from transgenic cows is one of the most interesting due to the high rhGH level achieved (5 g/L). We have designed and synthesized short peptides (9 or 10 amino acid long) using Fmoc chemistry and studied their ability to purify rhGH from milk once immobilized on an agarose support. Using spiked mi...
Recombinant human growth hormone (rhGH) is used for the treatment of several pathologies, most of them related to growth. Although different expression systems can be used for its production, the milk from transgenic cows is one of the most interesting due to the high rhGH level achieved (5 g/L). We have designed and synthesized short peptides (9 or 10 amino acid long) using Fmoc chemistry and studied their ability to purify rhGH from milk once immobilized on an agarose support. Using spiked milk with the hormone as a sample, rhGH was purified with 88% yield and 92% purity in a single step with a fold purification of 4.5.
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Key Words
DOWNSTREAM PROCESSINGTRANSGENIC COW MILKBIOLOGICSSOLID-PHASE PEPTIDE SYNTHESISRECOMBINANT BIOPHARMACEUTICALSPEPTIDE LIGANDS
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