Producción CyT
Artículo
Autoría
LIZARRAGA, MARIA ALFONSINA
;
Mola, Rocio
;
Lombardo, Emiliano
;
Maschi, Fabriico
;
Ayala, Miguel
;
RUMBO, MARTIN
;
GIOVAMBATTISTA, GUILLERMO
Fecha
2024
Editorial y Lugar de Edición
Buenos Aires-Argentina
Revista
INFO AACyTAL
(pp. 33-38)
- ISSN 2718-7683
Buenos Aires-Argentina
Buenos Aires-Argentina
ISSN
2718-7683
Resumen
Información suministrada por el agente en
SIGEVA
In order to reduce the number of used experimental animals and obtain a larger number of embryos per female, a superovulation protocol was improved using a sunflower supplementation. The superovulated was carried out using two hormones in 10 adult females of the B6J strain: equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG) intraperitoneally. At the time of administering hCG, each female was mated with a male. The females were distributed into two groups; one feeding wit...
In order to reduce the number of used experimental animals and obtain a larger number of embryos per female, a superovulation protocol was improved using a sunflower supplementation. The superovulated was carried out using two hormones in 10 adult females of the B6J strain: equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG) intraperitoneally. At the time of administering hCG, each female was mated with a male. The females were distributed into two groups; one feeding with Gepsa diet plus sunflower seeds supplementation and the other Gepsa diet without supplementation. The statistical analysis was performed using a generalized linear mixed model implemented in the software SAS. The quantity and quality of the embryos were evaluated in each experimental group through the cleavage rate, and the number expanded and hatched blastocysts based on the embryos obtained for each female. he obtained results did not show significance in the number of embryos between groups (p = 0.618). However, the supplemented group evidenced a higher number of cleavage rate (p = 0.002), the expanded blastocysts (p = 0.023) and hatched (p=0.029). These results could be explained because Sunflower seed is rich in omega 3 and has high energy, being a good supplement to improve the viability of embryos and the birth rate in rodents.
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Palabras Clave
Superovulaciónviabilidad embrionariaSemillas de girasolB6J
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