El efecto de la semilla de girasol en la dieta en embriones obtenidos mediante superovulación en B6J

Article

Authorship

LIZARRAGA, MARIA ALFONSINA ; Mola, Rocio ; Lombardo, Emiliano ; Maschi, Fabriico ; Ayala, Miguel ; RUMBO, MARTIN ; GIOVAMBATTISTA, GUILLERMO

Date

2024

Publishing House and Editing Place

Buenos Aires-Argentina

Magazine

INFO AACyTAL (pp. 33-38) - ISSN 2718-7683
Buenos Aires-Argentina

ISSN

2718-7683

Summary Information provided by the agent in SIGEVA

In order to reduce the number of used experimental animals and obtain a larger number of embryos per female, a superovulation protocol was improved using a sunflower supplementation. The superovulated was carried out using two hormones in 10 adult females of the B6J strain: equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG) intraperitoneally. At the time of administering hCG, each female was mated with a male. The females were distributed into two groups; one feeding wit... In order to reduce the number of used experimental animals and obtain a larger number of embryos per female, a superovulation protocol was improved using a sunflower supplementation. The superovulated was carried out using two hormones in 10 adult females of the B6J strain: equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG) intraperitoneally. At the time of administering hCG, each female was mated with a male. The females were distributed into two groups; one feeding with Gepsa diet plus sunflower seeds supplementation and the other Gepsa diet without supplementation. The statistical analysis was performed using a generalized linear mixed model implemented in the software SAS. The quantity and quality of the embryos were evaluated in each experimental group through the cleavage rate, and the number expanded and hatched blastocysts based on the embryos obtained for each female. he obtained results did not show significance in the number of embryos between groups (p = 0.618). However, the supplemented group evidenced a higher number of cleavage rate (p = 0.002), the expanded blastocysts (p = 0.023) and hatched (p=0.029). These results could be explained because Sunflower seed is rich in omega 3 and has high energy, being a good supplement to improve the viability of embryos and the birth rate in rodents.