Producción CyT
Unraveling the Role of UilS, a urea-induced acyl-homoserine lactonase that enhances Serratia marcescens fitness, interbacterial competition and urinary tract infection
Artículo
Autoría:
Tuttobene, Marisel R. ; Arango Gil, Brayan S ; Di Venanzio, G. ; Mariscotti, Javier F. ; Sieira, Rodrigo ; Feldman, M.F. ; Ramirez, Maria Soledad ; GARCIA VESCOVI, ELEONORAFecha:
2024Editorial y Lugar de Edición:
ASM PressRevista:
mBio - ISSN 2150-7511ASM Press
ISSN:
2150-7511Resumen *
Serratia marcescens, a member of theEnterobacteriaceae family, is an opportunistic human pathogen and frequentcause of urinary tract infections. Clinical isolates often exhibit resistanceto multiple antibiotics, posing challenges for successful treatment.Understanding its pathogenic mechanisms is crucial for elucidating newpotential targets to develop effective therapeutic interventions and manage S.marcescens infections. This work identifies UilS, a lactonase encoded inthe S. marcescens RM66262 strain isolated from a patient with a urinarytract infection. The study explores the bacterium´s response to urea, a majorcomponent of urine, and its impact on uilS expression. We found thatUilS degrades acyl-homoserine lactones (AHL) autoinducers traditionallyassociated with quorum sensing (QS) mechanisms. Surprisingly, UilS is able todegrade self and non-self AHL, exhibiting quorum-quenching activity towards Pseudomonasaeruginosa. We found that LuxR regulates uilS expression that isenhanced in the presence of AHL. In addition, urea-dependent induction of UilSexpression is controlled by the transcriptional response regulator CpxR. UilSconfers fitness advantage to S. marcescens, especially in the presenceof urea, emphasizing the adaptive plasticity of strains to modulate geneexpression based on environmental signals and population density. We alsodiscovered a novel bacterial killing capacity of S. marcescens thatinvolves UilS, indicating its importance in the interspecies interaction ofSerratia. Finally, we found that a uilS mutant strain displays attenuatedcolonization in a mouse model of catheter-associated urinary tract infection. uilSis present in clinical but absent in environmental isolates, suggesting anevolutionary adaptation to host-specific selective pressures. Información suministrada por el agente en SIGEVAPalabras Clave
pathogenesislactonaseQuorum quenchingSerratia