Producción CyT
LI Reunión Anual SAB - Prebiotics improved growth and hypocholesterolemic effect of the probiotic strain Limosilactobacillus reuteri CRL 1098
Congreso
Fecha:
2024Editorial y Lugar de Edición:
SABResumen *
The field of cardiovascular health management now emphasizes the potential benefits of synbiotic combinations comprising both probiotics and prebiotics. Limosilactobacillus (L.) reuteri CRL1098 is a probiotic bacterium known for its hypocholesterolemic effects linked to its bile salt hydrolase (BSH) enzyme. The purpose of the present study was to investigate the potential benefit of different prebiotics on bacterial growth, BSH activity and bile acid tolerance of CRL 1098 strain for the design of a synbiotic with hypocholesterolemic effect.To achieve this goal, MRS medium was supplemented with different prebiotics, including inulins with varying degrees of polymerization (FOS-P95, GR, HSI, and HP) and lactulose, at final concentrations ranging from 0.5% to 2.0% (w/v). Our results showed that the addition of lactulose improved probiotic growth respect to glucose with relative growth rates in a range of 1.7 to 2.5, while any inulins modified the strain growth. Remarkably, all prebiotics increased the BSH activity of the strain, thus enhancing its probiotic properties. The best results were observed with the addition of 2% which increased the activity by 20%, reaching almost 90% hydrolysis. However, prebiotics do not improve the survival of probiotics in the presence of bile acids. Our results showed that lactulose was able to promote probiotic growth and also have benefits on BSH activity which may ultimately positively modulate cholesterol metabolism. However, if deconjugation of bile salts with a view to lowering serum cholesterol is the main objective of the synbiotic formulation, inulin-type prebiotics can be considered, as even at low concentrations all were able to enhance BSH activity. Información suministrada por el agente en SIGEVAPalabras Clave
Prebiotics Limosilactobacillus reuteri CRL 1098hypocholesterolemic effect