Science and Technology Production
Congress
Authorship
Espariz, M
;
Mortera, P
;
BLANCATO, VICTOR SEBASTIAN
;
Suárez, CA
;
Repizo, GD
;
Alarcón, S
;
Magni, C
Date
2010
Publishing House and Editing Place
Biocell
ISSN
0327.9545
Summary
Information provided by the agent in
SIGEVA
Enterococcus faecalis is able to use malate in aerobic and anaerobic conditions. The mae locus of E. faecalis consist of two putative divergent operons, maePE and maeKR. The first operon codifies for the malic enzyme (maeE) and for the malate transporter (maeP). The second operon encodes the two component system (TCS), composed by the membrane kinase sensor (maeK) and the transcriptional regulator (maeR). In a previous work we showed that inactivation of the maeR or maeE caused complete loss of...
Enterococcus faecalis is able to use malate in aerobic and anaerobic conditions. The mae locus of E. faecalis consist of two putative divergent operons, maePE and maeKR. The first operon codifies for the malic enzyme (maeE) and for the malate transporter (maeP). The second operon encodes the two component system (TCS), composed by the membrane kinase sensor (maeK) and the transcriptional regulator (maeR). In a previous work we showed that inactivation of the maeR or maeE caused complete loss of malate utilization in E. faecalis. In this communication we demonstrated that both operons are specifically induced in response to the presence of malate in the growth medium and are repressed by the addition of PTS sugars. We performed transcriptional and mutagenesis analysis and established that a unique cre site (catabolite- responsive element) located between the -14 and -27 from maePE and -76 and -89 from maeKR transcriptional start sites governed the glucose repression of both operons.
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Key Words
catabolix repression