Science and Technology Production
Revista - Inhibition of Percupine has a differential impact on the gene expression signature of breast cancer cell lines of different levels of agrressiveness
Congreso
Authorship:
Sofia Valla ; Gianina Demarchi ; Nadia Bonadeo ; Agustina Chimento ; Lucia Romano ; ALANIZ, LAURA DANIELA ; Martin GötteDate:
2019Publishing House and Editing Place:
Fundación Revista MedicinaISSN:
1669-9106Summary *
Aggressiveness of the BC cell lines, we analyzed the impact of IWP-2, a PORCN inhibitor, on the TNBC cell line MDA-MB-231 and in the less aggressive MCF-7 cell line. Wnt pathway is involved in cellular processes which are dysregulated in cancer as cell renewal, proliferation and EMT. In particular, in breast cancer (BC) it has a role in both tumor initiation and progression. Porcupine?s palmitoylation of Wnt ligands is required for their proper signaling and release and its inhibition showed anti-tumoral effects on different BC models. In order to study if the inhibition of Porcupine (PORCN) had different effects regarding to the aggressiveness of the BC cell lines, we analyzed the impact of IWP-2, a PORCN inhibitor, on the TNBC cell line MDA-MB-231 and in the less aggressive MCF-7 cell line. Our results show that PORCN inhibition with IWP-2 induced a different expression signature of Wnt pathway, Cell Cycle and EMT related genes in both cell lines, although it was similar regarding Stem Cell markers. Moreover, in both cell lines it reduced β-CATENIN protein levels. The stronger effects observed in CFA and Cell Viability in MDA-MB-231 cells could be explained by changes in other cellular processes as apoptosis. These results suggest that the differences in the genetic background and phenotype of breast cancer cells can modulate Information provided by the agent in SIGEVAKey Words
WNT PORCN