Science and Technology Production
Book Chapter
Authorship
PERASSOLO, MARIA
;
Cardillo, Alejandra Beatriz
;
Busto, Víctor Daniel
;
Riviere, Stéphanie
;
Cerezo, Julieta
;
Giulietti, Ana María
;
Rodríguez Talou, Julián
Date
2020
Publishing House and Editing Place
Springer Nature Singapore Pte Ltd.
Book
Hairy Root Cultures (HRCs) based Applications -Methods and Protocols
Springer Nature Singapore Pte Ltd.
Springer Nature Singapore Pte Ltd.
ISBN
978-981-15-4055-4
Summary
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SIGEVA
Plant-derived drugs represent a significant proportion of the pharmaceutical market. Plant in vitro culture has emerged as a useful platform for producing secondary metabolites due to its multiple advantages: production under controlled culture conditions, independence from geographic, seasonal or weather conditions, and since it avoids the use of land and the extractionfrom the natural source, it has a low environmental impact. Since the production of secondary metabolites in plant in vitro cu...
Plant-derived drugs represent a significant proportion of the pharmaceutical market. Plant in vitro culture has emerged as a useful platform for producing secondary metabolites due to its multiple advantages: production under controlled culture conditions, independence from geographic, seasonal or weather conditions, and since it avoids the use of land and the extractionfrom the natural source, it has a low environmental impact. Since the production of secondary metabolites in plant in vitro cultures can be low, there are several strategies that can be applied to enhance the production of these compounds. Elicitation has been widely used to increase secondary metabolite production in plant in vitro cultures. This phenomenon involves the treatment of plantcultures with different agents (physical, chemical or biological) that triggers a defence response, which generally involves the production of secondary metabolites. Anthraquinones are secondary metabolites traditionally used as dyes that exhibit interesting therapeutic applications, such as antiviral (against Hepatitis C virus), and as photosensitizers of cancer cells. In this chapter, we will present an approach for the establishment of hairy root cultures of Rubia tinctorum, and a protocol for elicitation with methyl jasmonate. We will also discuss the possibility of combine elicitation with other strategies, such as in situ removal with Miglyol® 812.
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Key Words
HAIRY ROOTSRUBIA TINCTORUMELICITATIONMIGLYOL 812ANTHRAQUINONEMETHYL JASMONATEIN SITU REMOVAL