Science and Technology Production
Article
Authorship
Jimena H Martínez
;
Velazquez Francisco
;
Hernán Burrieza
;
MARTINEZ, KARINA DAFNE
;
Dominguez Rubio Paula
;
María del Pilar Buera
;
Oscar Perez
Date
2018
Publishing House and Editing Place
ELSEVIER SCI LTD
Magazine
FOOD HYDROCOLLOIDS
ELSEVIER SCI LTD
Summary
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The objective of the present contribution was to design and characterize betanin (Bt) loaded in a nanovehicle of 11Squinoa seed protein. 11S was isolated from quinoa seed floor. Protein purification was performed by Size-Exclusion Chromatography. MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization-Time-Of- Flight ) analysis confirmed the identity of 11S. Nanocarriers (11S-Bt) were generated at pH 8 at different ionic strength. Globulin intrinsic fluorescence spectra showed a quenching effect...
The objective of the present contribution was to design and characterize betanin (Bt) loaded in a nanovehicle of 11Squinoa seed protein. 11S was isolated from quinoa seed floor. Protein purification was performed by Size-Exclusion Chromatography. MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization-Time-Of- Flight ) analysis confirmed the identity of 11S. Nanocarriers (11S-Bt) were generated at pH 8 at different ionic strength. Globulin intrinsic fluorescence spectra showed a quenching effect exerted by Bt, demonstrating in turn protein-bioactive interaction. Stern -Volmer and Scatchard models application confirmed static quenching and allow obtaining parameters that described 11S and betanincomplexation process. Bt-11S globulin interactions seem to be more probably of physical type . Protein solubility was increasedafter complexation with Bt. 11S betanin-loaded nanocarrier showed additive effect in terms of both, antiradical or reducing power capacity in comparison to Bt as evaluated by two methods, 2,2 -azino-bis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS), and by ferric reducing antioxidant power (FRAP). Interestingly 11S globulinquaternary structure was modified by the bioactive, experimenting hexamer dissociation. This nanocolloid could have the potentiality to exert the Bt controlled delivery for pharmaceutical and nutraceutical products. Bt could also beprotected from light and oxygen in such systems.
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Key Words
BETATIN11S QUINOA GLOBULINNANOCARRIERANTIOXIDANT ACTIVITY