Science and Technology Production

Matrix assisted ultraviolet laser desorption/ionization time-of-flight (UV-MALDI-TOF) mass spectra of N-acylated and N,O-acylated glycosylamines

Articulo

Authorship:

Yasuto Sato ; Yuko Fukuyama ; Hiroshi Nonami ; Rosa Erra-Balsells ; Carlos A. Stortz ; Alberto S. Cerezo ; MATULEWICZ, MARIA CRISTINA

Date:

2007

Publishing House and Editing Place:

Elsevier Ltd.

Magazine:

CARBOHYDRATE RESEARCH, vol. 342 (pp. 2567-2574) Elsevier Ltd.

Summary *

Ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (UV-MALDI-TOF-MS) has shown to be a very useful technique for the study of the non-volatile and thermally non-stable N-acylated glycopyranosyl- and glycofuranosyl-amines. Of the several matrices tested, 2,5-dihydroxybenzoic acid (DHB) was the most effective giving good spectra in the positive-ion mode. In the linear and reflectron modes the [M + Na]+ ions appeared with high intensity. Their fragmentation patterns were investigated by post-source decay (PSD) UV-MALDI-TOF-MS showing mainly cross-ring cleavages. In addition, N,O-acylated glycopyranosyl- and glycofuranosyl-amines were also analyzed by this technique. PSD UV-MALDI-TOF-MS gave significant signals for several primary fragment ions which were proposed but not detected, or observed with very low abundance, in electron ionization mass spectrometry (EI-MS) experiments. Information provided by the agent in SIGEVA