Anthocyanin-Enriched Extract from Ribes nigrum Inhibits Triglyceride and Cholesterol Accumulation in Adipocytes

Article

Authorship

Montaldo, Laura Andrea ; Gallo, Alicia ; Rocha, Gabriela Fernanda ; Csernoch, Cecilia Edith ; DE MARZI, MAURICIO CESAR ; GUERRA, LILIANA NOEMI

Date

2023

Publishing House and Editing Place

Newlands Press

Magazine

Therapeutic Delivery, vol. 14 (pp. 675-687) - ISSN 2041-5990
Newlands Press

ISSN

2041-5990

Summary Information provided by the agent in SIGEVA

Aim: Obesity is a chronic pathology of epidemic proportions. Mature adipocytes from a 3T3-L1 cell line were used as in vitro obesity model to test different bioactive compounds. We aim to evaluate cassis (Ribes nigrum) extract antioxidant activity and its antiadipogenic effect on mature adipocytes. Results: We produced an extract by using enzyme that combines cellulase and pectinase; we obtained high yield of the bioactive compound anthocyanin. Extract showed high antioxidant capacity. We condu... Aim: Obesity is a chronic pathology of epidemic proportions. Mature adipocytes from a 3T3-L1 cell line were used as in vitro obesity model to test different bioactive compounds. We aim to evaluate cassis (Ribes nigrum) extract antioxidant activity and its antiadipogenic effect on mature adipocytes. Results: We produced an extract by using enzyme that combines cellulase and pectinase; we obtained high yield of the bioactive compound anthocyanin. Extract showed high antioxidant capacity. We conducted in vitro assays by adding the extract to adipocytes culture medium. Extract reduced intracellular levels of triglyceride by 62% and cholesterol by 32%. Conclusion: Enzymatic extract’s high antioxidant activity was likely attributable to its high concentration of anthocyanin. This extract inhibits lipid accumulation in adipocytes.