Science and Technology Production
A new approach to lysozyme in cheese manufacture: boosting key enzyme activities in ripening process.
Article
Authorship:
Peralta, Guillermo H. ; Bürgi, Milagros ; Albarracín, Virginia H. ; Sanches, Renzo ; Martínez, Luciano J. ; BERGAMINI, CARINA VIVIANADate:
2024Publishing House and Editing Place:
CAMBRIDGE UNIV PRESSMagazine:
Journal of Dairy Research CAMBRIDGE UNIV PRESSSummary *
The rational use of lactobacilli and lysozyme (LZ) could be a simple strategy boosting key enzyme activities during cheese ripening. This study investigated the effect of the application of LZ on the cell envelopes and metabolic potential of two strains of mesophilic lactobacilli: Lactiplantibacillus plantarum 29 (L29) and Lacticaseibacillus rhamnosus 77 (L77). An in vitro study about LZ-sensitivity was carried out on the two strains. The results of turbidity, culturability, physiological profile as well as fluorescence microscopy images, and scanning electron micrographs confirmed in L29 a high-level of sensitivity to LZ. Therefore, L29 alone or in combination with LZ as well as a cell-free extract of L29 were used as ripening agents in the manufacture of a mature cheese. The release of peptidases in the cheese matrix by the lysis of L29 in situ was validated and associated with positive effects on the cheese quality, especially the increase of hydrophilic peptide levels. Information provided by the agent in SIGEVAKey Words
LACTIC ACID BACTERIALYSOZYMEENZYMESRIPENINGCHEESE