Producción CyT
Congreso
Autoría
Hech Evelin sabrina
;
Sanmarco Liliana
;
Tjon Emily
;
Preciado Maria victoria
;
Correale Jorge
;
Quintana Francisco Javier
;
LORENZETTI, MARIO ALEJANDRO
;
Farez Mauricio Franco
Fecha
2021
Editorial y Lugar de Edición
Sociedad Argentina de Investigación Clínica
Resumen
Información suministrada por el agente en
SIGEVA
Multiple Sclerosis (MS) is a demyelinating, chronic, autoimmune disease that affects the central nervous system (CNS). Is the leading cause of non-traumatic neurological disability among young adults and there is no definitive treatment for it. Its etiology is not absolutely clear, but Herpesvirus infection, Epstein Bar virus (EBV) in particular, has been suggested as an environmental factor associated with MS risk and severity. We sought to compare antibody (Ab) titers against EBV, Cytomegalov...
Multiple Sclerosis (MS) is a demyelinating, chronic, autoimmune disease that affects the central nervous system (CNS). Is the leading cause of non-traumatic neurological disability among young adults and there is no definitive treatment for it. Its etiology is not absolutely clear, but Herpesvirus infection, Epstein Bar virus (EBV) in particular, has been suggested as an environmental factor associated with MS risk and severity. We sought to compare antibody (Ab) titers against EBV, Cytomegalovirus (CMV) and Varicella Zoster virus (VZV) between MS patients and healthy controls (HC) from Argentina and to characterize EBV polymorphisms associated with disease risk.Serology was assessed in 146 MS patients and 129 HC by indirect ELISA (Vircell).In a subset of MS cases, EBV EBNA-2 and vIL-10 genes were amplified and Sanger sequenced and RNAseq was performed in EBV+ and EBV- sorted B cells.All MS patient and HC were IgG positive for EBV and VZV, while 60% of the HC and 71% of the MS patient were seropositive for CMV. A significant difference was found between HC and MS patients for EBV-VCA titer (P<0.01) and CMV Ab titer (P< 0.05). RNAseq of cell-sorted EBV+ B cells showed down-regulation in IQGAP1 mRNA, a B cell proliferation regulator molecule, in comparison to EBV- B cells. Sequencing of EBNA-2 revealed a CTC insertion at nucleotide position 633 in 5/8 MS samples but not in HC (0/4). No MS specific polymorphisms were found in vIL-10 gene (12 MS samples vs 8 HC).MS is a complex disease with strong interaction between genetic and environmental factors, and different Herpesviruses may have an effect on MS pathology, as suggested by differences in titers for EBV-VCA and CMV Ab, RNAseq results and an EBV EBNA2 gene variant potentially linked to MS patients. Further work is needed to validate sequencing results and confirm this association.
Ver más
Ver menos
Palabras Clave
HERPESVIRUSVIRUS EPSTEIN BARRESCLEROSIS MULTIPLECITOMEGALOVIRUS