1. CONICET
  2. Producciónes CyT
Producción CyT
Molecular detection of bee pathogens in honey

Artículo

Autoría
MEDICI, SANDRA KARINA
Fecha
2020
Editorial y Lugar de Edición
Wageninen Academy
Revista
Journal of Insects as Food and Feeds - ISSN 2352-4588
Wageninen Academy
ISSN
2352-4588
Resumen Información suministrada por el agente en SIGEVA
Honey bees are affected by a wide variety of parasites and pathogens, some of them werepreviously found in bee honey. Considering that spore-forming microorganisms are expected toremain latent in raw honey, presence of microsporidia, spore-forming bacteria, and viruses protected by peptide structures might represent a threat for bees. It is widely known that parasites andpathogens are one of the main factors implied in the worldwide decline of wild pollinators andcolony losses of managed bees, ... Honey bees are affected by a wide variety of parasites and pathogens, some of them werepreviously found in bee honey. Considering that spore-forming microorganisms are expected toremain latent in raw honey, presence of microsporidia, spore-forming bacteria, and viruses protected by peptide structures might represent a threat for bees. It is widely known that parasites andpathogens are one of the main factors implied in the worldwide decline of wild pollinators andcolony losses of managed bees, and their detection in honey could be used to prevent the spread ofdiseases among colonies. Honey from 57 apiaries of Argentina located in Buenos Aires, Córdoba,Corrientes, Entre Ríos, Formosa, La Rioja, Neuquén, Río Negro and Santa Fe provinces was collected between March and October, 2012. DNA was extracted from the pellet obtained by centrifugation of 10 g of honey and pathogen DNA was amplified by qPCR, and products were purified,sequenced and analysed using BLAST software. Honey from every apiary contained DNA of atleast one pathogen, with a high occurrence of Apis mellifera Filamentous Virus (AmFV) andApicystis bombi, although Nosema ceranae, Paenibacillus larvae, and Ascosphaera apis were alsodetected. Here, we report the presence of DNA of several bee pathogens in honey from commercialapiaries, and provide a fast and efficient screening method that could be useful to indirectly estimate pathogen presence in apiaries.
Ver más Ver menos
Palabras Clave
food analysisPCRdna virusmicrosporidia