Producción CyT

Journal of Animal Science - Effect of dietary fish oil and vitamin E on DNA damage in dogs undergo training.

Congreso

Autoría:

Risso A ; Pellegrino F ; Corrada Y ; Nikoloff N ; SEOANE, ANALIA ISABEL ; Relling A

Fecha:

2017

Editorial y Lugar de Edición:

American Society of Animal Science

ISSN:

0021-8812

Resumen *

The aim of the present study was to evaluate the effect of dietary supplementation with fish oil (FO) alone or FO and vitamin E (VE) on DNA damage and serum VE concentration in dogs undergoing training. Using a replicate 3x3 Latin square design: 1) a control diet (CG); 2) the same diet supplemented with 54 mg FO/kg BW (FG); and 3) a similar diet plus FO (54mg/kg BW) and 400 mg VE (FEG) per day for 60 d. Blood samples were collected on d0, 30 and 60. Deoxyribonucleic acid damage was measured using a single-cell gel electrophoresis assay in peripheral whole blood leukocytes. Deoxyribonucleic acid damage was classified in 4 classes (I = undamaged; II = minimum damage, III = medium damage and IV = maximum damage). Dogs were trained on a treadmill at 8 km/h and 7.5% slope twice a week for 60 d. Each session lasted 30 min. Data were analyzed usin PROC MIXED of SAS (9.4 SAS Inst. Inc., Cary, NC). Treatment time, and their interaction were considered fixed variables and dog and period were considered random. The SLICE option of SAS was used for mean separation when there was a treatment x time interaction. On d 30, there was a significant increase in DI in FG compared with CG and FEG (p<0.01). DNA damage index values were 0.0002, 0.105, 0.109, 0.053, 0.743, 0.467, 0.175, 0.127, and 0.193 for CG, FG and FEG ond0, 30 and 60, respectively. There was a time x treatment interaction for the serum VE concentration (p<0.01). On d 0 and on d30, there were no differences in serum VE concentration of the 3 groups (p<0.1). However, on d 60, serum VE concentration were higher in FEG compared with those in CG and FG (6.200 vs. 2.300 vs. 2.000 µg/d. respectively; p<0.01). In conclusion, FO supplementation produces DNA damage after30 d but not at 60 d. The supplementation of FO with VE could prevent the damage at 30 d. Supplementation with VE increases serum VE concentration Información suministrada por el agente en SIGEVA

Palabras Clave

FISH OILDOGSDNA DAMAGEVITAMIN E