Producción CyT

XVIII Brazil MRS Meeting - Nanoparticle colloidal stability investigated by small-angle x-ray scattering

Congreso

Autoría:

Flavia E. Galdino ; PICCO, AGUSTIN SILVIO ; Renata Lang Sala ; Mateus Borba Cardoso

Fecha:

2020

Editorial y Lugar de Edición:

SBPMat

ISSN:

978-85-63273-40-6

Resumen *

An evaluation of nanoparticle colloidal stability in the presence of protein isessential to understand the nanoparticle behavior in a biological medium.Frequently, dynamic light scattering (DLS) is the main technique used to makethis kind of evaluation. However, we have found out that small-angle X-rayscattering (SAXS) is also an interesting technique to study colloidal stability and protein corona. The SAXS results were evaluated by the variation in theextrapolated intensity at zero scattering angle [I (0)] of the nanoparticles as a function of protein concentration. This work uses these two techniques to studythe adsorption of bovine serum albumin (BSA) and fetal bovine serum (FBS) on bare silica nanoparticles (Bare) and silica nanoparticle-modified by zwitterionic(ZW) and polyethylene glycol (PEG). While the hydrodynamic diameter of thenanoparticles shifted to smaller values due to the contribution of free proteins on DLS results, the evaluation of protein corona by SAXS did not have this artifact.In addition, the SAXS technique was able to detect a thin corona protein that did not affect the colloidal stability, as well a system of pair of nanoparticles having interaction, as confirmed by cryo-em. Información suministrada por el agente en SIGEVA

Palabras Clave

COLLOIDAL STABILITYSILICA NANOPARTICLESDLSSAXS