Producción CyT
1st QST International Symposium: ?Quantum Life Science? - Quantum Biology of Reactive Oxygen Species and its Impact in Cellular Bioenergetics
Congreso
Fecha:
2017Editorial y Lugar de Edición:
QST International Symposium SecretariatResumen *
QuantumBiology of Reactive Oxygen Species and its Impact in Cellular Bioenergetics Pablo R Castello1,Christina Chavarriaga2, Maria Procopio3, and Carlos F.Martino2 1. Facultad de Ciencias Exactas y Naturales. Universidadde Belgrano, CABA, Argentina 2. Department of Biomedical Engineering,Florida Institute of Technology, Melbourne, FL 32901 3. Department of Biophysics, Johns HopkinsUniversity, Baltimore, MD 21218 cmartino@fit.edu QuantumBiology (QB) may be thought of as the signatures of molecular-level quantumphenomena observed in biological systems at the functional, cellular, ororganism levels. For example, quantumeffects in biological systems have been implicated in mechanisms for birdnavigation, olfactory sensing, and photosynthesis. The authors depart from these manifestlyphenomena of QB and present an innovative approach that reveals a novel domainof quantum biology: the control of biological production of reactive oxygenspecies via coherent spin dynamics in a radical pair reaction. The broad aim is to advance our understandingof how the spin biochemistry involved in flavin semiquinone (FADH?)and superoxide anion (O2?-) radical pair formation (FADH?:O2?-)and singlet-triplet intersystem crossing can be manipulated, inflavoenzymes, to influence ROS products and ROS levels. The talk is organized into two interrelated studies: 1) study at the whole cellular level of light-independent mechanisms for normalcellular metabolism whereby spin-correlated radical pairs mediate the biologicalproduction of ROS. The radical pairmechanism is studied by applying radio frequency (RF) magnetic fields andlow-level static magnetic fields (LLF) to cell cultures and the responsecharacteristics of ROS production and cellular bioenergetics are assayed; 2)time-dependent quantum mechanics formalism is applied to corroborateexperimental magnetic field and frequency resonances that influence quantumstates in non-equilibrium biological systems. Specifically, intracellular O2?- and extracellularhydrogen peroxide (H2O2) were investigated in vitro alongwith respiration and glycolysis with primary human umbilical vein endothelialcells (HUVECs). Theoretical analysis considers RF and LLF magnetic fieldeffects in a one-proton radical pair model. HUVECs were exposed to either 50 μTstatic magnetic fields (SMF), or to static magnetic fields combined with 1.4 or7 MHz RF magnetic fields in parallel or perpendicular corresponding to Zeemanand hyperfine interactions, respectively. We observe differential changes inbioenergetics and in consumption of O2?- and production H2O2as a function of angle between SMF and RF magnetic fields. The orientationeffects that lead to specific ROS product distribution is consistent with the spinbiochemistry model; however, we do not know the specific spin biochemistry targetsor signaling channels that lead to changes in bioenergetics. We propose that O2?-and H2O2 production in metabolic processes occur throughsinglet-triplet modulation of FADH? enzymes and O2?- spin-correlatedradical pairs. Spin-radical pair products are modulated by the RF magneticfields that presumably decouple flavin hyperfine interactions during spincoherence. RF flavin hyperfine decoupling results in changes of H2O2singlet state products, which creates cellular oxidative stress and acts as asecondary messenger that affects bioenergetics. This study demonstrates the interplaybetween O2?- and H2O2 productionwhen influenced by RF magnetic fields and underscores the subtle effects oflow-frequency magnetic fields on oxidative metabolism, ROS signaling, andcellular growth. Información suministrada por el agente en SIGEVAPalabras Clave
magnetic fields Quantum Biology Spin-radical pair Reactive Oxygen Species