Producción CyT
Sociedad Argentina de Biología - Antimicrobial and anti-inflammatory effects of dodecenyl succinic anhydride modified collagen hydrogels loaded with simvastatin.
Congreso
Autoría:
Olivetti, Christian Ezequiel ; ALVAREZ, GISELA SOLANGE ; Perna Oriana ; Mitarotonda Romina ; De Marzi Mauricio ; Desimone Martin FedericoFecha:
2018Editorial y Lugar de Edición:
SABResumen *
Collagen dressings have been extensively used for wound healing as they restore the barrier function lost during injury, while stimulating cell migration and supporting the growth of new cells in the affected area. Nevertheless, collagen scaffolds often present poor mechanical properties, high susceptibility to degradation and low capacity to incorporate hydrophobic drugs. In order to improve their properties, physical and chemical modifications are necessary. Herein, we developed a novel hydrophobic collagen wound dressing to incorporate simvastatin, which has potential application in the treatment of ulcers and prevention of wound infection. For that matter, collagen hydrogels were grafted with dodecenylsuccinic anhydride (DDSA). Solid NMR was performed in order to confirm that DDSA was covalently bound to hydroxyl or amine groups of collagen amino acids. Also, an increase in the water contact angle was observed, providing good evidence of the chemical modification proposed for the reactive, and the incorporation of hydrophobic domains. Modified hydrogels were loaded with simvastatin showing higher adsorption capacity and lower release. Lastly, the antimicrobial and anti-inflammatory activities of DDSA-collagen materials were assessed. DDSA-collagen hydrogels, either unloaded or loaded with simvastatin showed sustained antimicrobial activity against Pseudomonas aeruginosa and Staphylococcus aureus for 72 h probably due to the hydrophobic interaction of DDSA chains with bacterial cell wall. The antimicrobial activity was stronger against S. aureus. Collagen hydrogels also presented a prolonged antibacterial activity when they were loaded with simvastatin, confirming the antimicrobial properties of statins. Finally, it was observed that these materials can stimulate resident macrophages and promote an M2 profile which is desirable in wound healing processes. Información suministrada por el agente en SIGEVAPalabras Clave
colágenoantiinflamatorioantimicrobianosimvastatina