Producción CyT
Artículo
Autoría
Fecha
2007
Editorial y Lugar de Edición
Blackwell
Revista
REPRODUCTION IN DOMESTIC ANIMALS (1990),
vol. 42
(pp. 423-426)
Blackwell
Resumen
Información suministrada por el agente en
SIGEVA
Laparoscopic ovum pick-up (LOPU) is a modern technique that may be used in programs of sheep and goats in vitro embryo production. The effects of repeated recoveries and follicular diameter on the efficiency of LOPU were evaluated in these species. In six Merino donor sheep and five Criolla goats follicle development was stimulated by gonadotrophins. The gonodotrophin treatment was repeated 4 times in sheep and 3 in goats, with 4 days interval. Follicular fluid was aspirated under laparoscopic ...
Laparoscopic ovum pick-up (LOPU) is a modern technique that may be used in programs of sheep and goats in vitro embryo production. The effects of repeated recoveries and follicular diameter on the efficiency of LOPU were evaluated in these species. In six Merino donor sheep and five Criolla goats follicle development was stimulated by gonadotrophins. The gonodotrophin treatment was repeated 4 times in sheep and 3 in goats, with 4 days interval. Follicular fluid was aspirated under laparoscopic observation according to the follicle diameter with a cannula for intrauterine artificial insemination without a vacuum pump. The number and size of follicles, oocyte quantity and quality and recovery rate were evaluated. A total of 566 follicles were aspirated (14.4±2.4 follicles/sheep and 14.6±7.5 follicles/goat) obtaining a total of 263 oocytes (6.8±1.5 oocytes/sheep and 6.6±2.9 oocytes/goat) and a recovery rate of 47% in sheep and 46% in goats. In both species, there were no significant differences in the number of aspirated follicles, recovered oocytes, recovery rate and good quality oocytes recovery rate in the repeated LOPU. The oocyte recovery rates in sheep were significantly different according to the follicular size (55% in large follicles, 36% in small follicles) (P<0.05), while in goats recovery rates of oocytes related to the follicular size showed no significant differences (42 and 54% for large and small follicles, respectively). Follicular sizes from which oocytes were obtained did not determine any significant variation in the recovery rate of good quality oocytes. The technique used would allow effective and repeated oocyte recoveries for in vitro embryo production in sheep and goats.
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Palabras Clave
Laparoscopic ovum pick-upOocyte collection