Producción CyT
Congreso
Autoría
Fecha
2017
Editorial y Lugar de Edición
Wiley Blackwell
ISSN
1439-0531
Resumen
Información suministrada por el agente en
SIGEVA
In the testis, smooth muscle-like peritubular cells build the wall of seminiferous tubules. Transport of immotile sperm to the rete testis has been regarded as their major role. We have established a culture procedure for human testicular peritubular cells (HTPCs) and a subsequent proteome-analysis of HTPCs has revealed among others cyclooxygenase 1 (COX1) and two PGE-synthases (PTGES2 and 3). This implies that HTPCs are a source of PGE2, which consequently may be a physiological factor in the ...
In the testis, smooth muscle-like peritubular cells build the wall of seminiferous tubules. Transport of immotile sperm to the rete testis has been regarded as their major role. We have established a culture procedure for human testicular peritubular cells (HTPCs) and a subsequent proteome-analysis of HTPCs has revealed among others cyclooxygenase 1 (COX1) and two PGE-synthases (PTGES2 and 3). This implies that HTPCs are a source of PGE2, which consequently may be a physiological factor in the human testis. Western blots confirmed that HTPCs express COX1 and ELISA-measurements showed that they constitutively secrete PGE2. Indomethacin, a prototype non-steroidal anti-inflammatory drug (NSAID), significantly reduced PGE2 synthesis. Immunohistochemistry (IHC) showed COX1 and specific receptors for PGE2, EP1-4 in human testicular samples. EP1, EP2 and EP4 were revealed in HTPCs in vivo and in vitro by RT-PCR and/or IHC. Results of collagen gel contraction assay indicate that PGE2 does not acutely contract HTPCs, yet qPCR studies showed that it increased the mRNA levels for the contractility proteins, smooth muscle actin (SMA) and calponin in a concentration-depended manner. HTPCs, like Sertoli cells, produce glial derived neurotrophic factor (GDNF), which regulates the renewal of spermatogonial stem cells (SSCs). PGE2 increased GDNF mRNA and GNDF levels in the culture medium. Thus, HTPCs are sources of PGE2, which may contribute to testicular homeostasis. This includes maintenance of the contractile phenotype of peritubular cells and, via GDNF, a fine-tuning of the SSC niche. Blockage of PG-production e.g. by NSAIDs, steroids or other substances may thus have unwanted testicular side effects. (Support: DFG MA1080/25-1)
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Palabras Clave
TESTISPGE2HOMEOSTASISPERITUBULAR CELLS