Producción CyT
BIOCELL - The Mce1 proteins of Mycobacterium tuberculosis are involved in the uptake and metabolism of lipids.
Congreso
Autoría:
FORRELLAD, MARINA ANDREA ; Klepp, LI ; Blanco, FC ; Bianco, MV ; Santangelo, MP ; Jackson, M ; Gutierrez, MG ; Bigi, FFecha:
2011Editorial y Lugar de Edición:
Sociedad Latinoamericana de Microscopía ElectrónicaISSN:
0327-9545Resumen *
The Mce proteins of M. tuberculosis have been implicated in the entry and persistency of the bacterium into the host cells. These proteins are codified in four locus in the M. tuberculosis genome: mce1, -2, -3 and -4, each one includes two yrbE and six mce genes. The in silico analysis have revealed the Mce proteins as transporter systems. Recently, it was demonstrated that Mce4 proteins are involved in cholesterol uptake. Due to, one adaptation of M. tuberculosis to persist into the host cells is its changes to utilizes lipids as carbon source, we analyzed the role of Mce1 proteins into the uptake and metabolism of palmitic acid. We have observed that M. tuberculosis mce1 mutant incorpotes less [14C]-palmitic acid compared to the wilt type (WT) in the first 30min of an uptake experiment. However, after 30min the [14C]-palmitic acid accumulates in the mutant while the wild type remains constant. In addition, by qRT-PCR experiment we observed a correlation between the uptake of palmitic acid and the mce1 genes expression. Also, a differential lipid profile was observed in the mutant compared to the WT, when the bacteria were grown in minimal medium with palmitic acid as carbon source. In this late condition, we identified two non-glicolipids accumulated in the mutant. In this study we also analysed the Mce1 proteins localization and the intracellular traffic of the mce1 mutant compered to the WT. Taken together, the results presented here suggest that the Mce1 proteins are a palmitic acid transporter system in M. tuberculosis and perhaps it would be related to the membrane lipids recycling. Información suministrada por el agente en SIGEVAPalabras Clave
M. tuberculosislipid metabolimsMce1