Functionalization of bamboo-like carbon nanotubes with 3-mercaptophenylboronic acid-modified gold nanoparticles for the development of a hybrid glucose enzyme electrochemical biosensor

Artículo

Autoría:

Eguílaz Rubio, Marcos ; Villalonga, Reynaldo ; Pingarrón, José M. ; FERREYRA, NANCY FABIANA ; RIVAS, GUSTAVO ADOLFO

Fecha:

2015

Editorial y Lugar de Edición:

Elsevier Science Sa

Revista:

SENSORS AND ACTUATORS B-CHEMICAL, vol. 216 (pp. 629-637) Elsevier Science Sa

Resumen

We report for the first time a sensitive and selective glucose biosensor based on the immobilization at glassy carbon electrodes (GCE) of a new hybrid nanomaterial consisting of gold nanoparticles functionalized with 3-mercaptophenyl boronic acid (AuNPs-B(OH)2) and bamboo-like multiwall carbon nanotubes (bMWCNT) dispersed in hyperbranched polyethyleneimine (PEI). The presence of boronic acid residues allowed the supramolecular immobilization of glucose oxidase (GOx) as a model glycoenzyme while the hybrid nanomaterial Au(NP)-bMWCNT produces a synergistic effect on the catalytic detection of the enzymatically generated hydrogen peroxide. The resulting functionalized nanomaterials were characterized by TEM, FT-IR and electrochemical techniques. The sensitivity at 0.700 V was (3.26 ± 0.03) mA M?1 (28.6 mA M?1 cm?2), with a linear range between 2.50 × 10?4 M and 5.00 × 10?3 M, a detection limit of 0.8 ?M and a quantification limit of 2.4 ?M. The biocatalytic layer demonstrated to be highly reproducible with R.S.D. values of 8.6% for 10 successive amperometric calibrations using the same surface, and 4.5% for ten different bioelectrodes. The sensitivity of the biosensor after 14 days of storage at 4 °C remained at 86.1% of its original value. The combination of the excellent dispersing properties of PEI, the stability of the bMWCNT-PEI dispersion, the synergistic effect of AuNPs-B(OH)2 and bMWCNT towards the electrooxidation of hydrogen peroxide, and the robust immobilization of GOx at AuNP-B(OH)2 allowed building a sensitive, reproducible and stable amperometric biosensor for the quantification of glucose in beverages and milk samples.

Palabras Clave

CARBON NANOTUBES DISPERSIONGOLD NANOPARTICLESBAMBOO-LIKE MULTIWALLED CARBON NANOTUBESBORONIC ACIDGLUCOSE OXIDASEPOLYETHYLENIMINEAMPEROMETRIC GLUCOSE BIOSENSOR

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http://hdl.handle.net/11336/44414