Producción CyT
Congreso
Autoría
Cáceres L
;
PAZ, MARIELA LAURA
;
Adami PM
;
Ojeda D
;
Garcés M
;
Calabró V
;
Tasat D
;
Beron AM
;
Alvarez S
;
González Maglio DH
;
Marchini T
;
Evelson PA
Fecha
2017
Editorial y Lugar de Edición
Fundación Revista Medicina
Resumen
Información suministrada por el agente en
SIGEVA
The exposure to environmental particulate matter (PM) is associated with pulmonary oxidative stress and inflammation. Alveolar macrophages are suggested to play a central role in this scenario, since they produce inflammatory mediators and reactive oxygen species. The aim of this work was to study the redox status and the role of mitochondria in macrophages after the exposure to Residual Oil Fly Ash (ROFA), a PM surrogate rich in transition metals. The murine cell line RAW 264.7 was exposed to ...
The exposure to environmental particulate matter (PM) is associated with pulmonary oxidative stress and inflammation. Alveolar macrophages are suggested to play a central role in this scenario, since they produce inflammatory mediators and reactive oxygen species. The aim of this work was to study the redox status and the role of mitochondria in macrophages after the exposure to Residual Oil Fly Ash (ROFA), a PM surrogate rich in transition metals. The murine cell line RAW 264.7 was exposed to ROFA at 100 μg/mL for 24 hs. Cell viability was not significantly affected under these experimental conditions. Intracellular redox status was assessed by flow cytometry as dichlorofluorescein mean fluorescence intensity (MFI), which was increased by 30% following ROFA incubation compared to the control group (p<0.05). Mitochondrial O2●- production was determined by MitoSox fluorescent probe, resulting in an increase of 167% (control: 9±4% MitoSox+ cells, p<0.05). Consistently, mitochondrial inner membrane potential decreased by 41% (control: 80±6% TMRM+ cells, p<0.05). Mitochondrial functionality was also assessed by Seahorse XF Analyzer, resulting in an 85% decrease in respiratory control ratio (p<0.05), as well as a 33% decrease in the ATP-O ratio (p<0.05), an indicator of oxidative phosphorylation efficiency, and a 2-fold increase in the H+ leak (p<0.01), an indicator of the mitochondrial inner membrane integrity, following ROFA incubation. Given that we observed a different response with Concentrated Ambient Particles (CAPs), another source of PM, which differs in elemental composition from ROFA, we suggest that PM chemistry might play an important role in the observed effects. These findings indicate that redox status of RAW 264.7 cell line is affected by the exposure to ROFA, probably due to changes in mitochondrial function.
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Palabras Clave
MACROPHAGESOXIDATIVE STRESSINFLAMMATIONAIR POLLUTION