Producción CyT
Artículo
Autoría
PAZ, MARIELA LAURA
;
Cela, Eliana Maiten
;
FERRARI, ALEJANDRO
;
Friedrich, Adrian D.
;
Leoni, Juliana
;
GONZALEZ MAGLIO, DANIEL HORACIO
Fecha
2014
Editorial y Lugar de Edición
Scientific Research Publishing
Revista
Open Journal of Rheumatology and Autoimmune Diseases,
vol. 4
(pp. 1-12)
- ISSN 2163-9914
Scientific Research Publishing
Scientific Research Publishing
ISSN
2163-9914
Resumen
Información suministrada por el agente en
SIGEVA
Background: Autoantibodies (Aabs) are the hallmark of numerous systemic autoimmune pathologies (SAPs), for instance anti-Ro/SS-A Aabs are usually found in Systemic Lupus Erythematosus (SLE) and Sjogren’s Syndrome. Cutaneous photosensitivity (CP) is found in most forms and subsets of LE and consists of a skin rash as a result of unusual reaction to sunlight. There are many theories which relate specifically the presence of circulating anti-Ro/SS-A Aabs with the CP phenomenon, though there ...
Background: Autoantibodies (Aabs) are the hallmark of numerous systemic autoimmune pathologies (SAPs), for instance anti-Ro/SS-A Aabs are usually found in Systemic Lupus Erythematosus (SLE) and Sjogren’s Syndrome. Cutaneous photosensitivity (CP) is found in most forms and subsets of LE and consists of a skin rash as a result of unusual reaction to sunlight. There are many theories which relate specifically the presence of circulating anti-Ro/SS-A Aabs with the CP phenomenon, though there are several studies which are in disagreement. Results: In this study we analyzed the relationship between CP and anti-Ro Aabs by means of two approaches. The first one included an in vitro model where we evaluated by flow cytometry the binding capacity of affinity-purified Aabs to autoantigens relocalized on apoptotic keratinocyte’s surface. We found that there was no relationship between the binding capacity of serum from 10 selected patients or their corresponding purified anti-Ro52 and anti-Ro60 Aabs, and the presence or absence of CP, neither with the SAPs. The in vivo model consisted of Hairless SKH:1 mice which were induced to produce anti-murine Ro52 and/or Ro60 Aabs and were subsequently irradiated with UVB light. We evaluated the skin histology and also the epidermal production of TNF-?. We found no differences between the groups in neither of the parameters evaluated. Conclusions: These results agree with some studies on the role of the Aabs in CP, considering anti-Ro Aabs not as the only responsible for the manifestation; and disagree with many other authors, who believe in the strong association between these two events.
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Palabras Clave
UVB IRRADIATIONSKH-1 HAIRLESS MICEPURIFIED ANTI-RO/SS-A AUTOANTIBODIESCUTANEOUS PHOTOSENSITIVITYTNF-alpha
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