Producción CyT

Applied research in toxicology - HIGH THROUGHPUT (RNA-SEQ) SCREENING FOR BIOMARKERS OF AZINPHOS METHYL (AZM) EXPOSURE IN Rhinella arenarum

Congreso

Autoría:

MARDIROSIAN, MARIANA NOELIA ; Ceschin Danilo ; Pires Natalia ; Lascano Cecilia ; Venturino Andres

Fecha:

2015

Editorial y Lugar de Edición:

Brasilian Society of Toxicology

ISSN:

2359-4721

Resumen *

Azinphos methyl (AZM) is the main organophosphate pesticide applied in Río Negro-Neuquén valley in Argentina. In this sense, it is necessary to control the levels of residues and environmental impact of pesticides. Traditional ecotoxicological biomarkers like reduced glutathione (GSH) levels and the activities of Glutathione S transferase (GST) and Catalase (CAT) are commonly used for biomonitoring. However, they may fail for sensitivity and unspecifically respond to oxidative stress. Native species is a preferred resource for its ecological significance. In this sense, we have evaluated pesticide effects on common toad R. arenarum larvae. In this work, we screen for molecular biomarkers using RNA-Seq technology and compare the response with traditional biomarkers, in order to develop new specific and sensitive biomarkers. We could detect changes only in GSH content but not in GST or CAT activities at 6h and 24h exposures to AZM. Regarding to the transcriptomic profile, we could determine up- and down-regulated genes compared with control for both 6h and 24h of exposure. After gene enrichment analysis, over representation of detoxification and oxidative stress pathways was found. Moreover, for Gene Ontology classification there were hits for Biological Process category such as cell differentiation, developmental growth, catabolic and hormone metabolic process, cell proliferation and cell cycle.Here we show that transcriptomic analysis is a good tool to pick up gene candidates to be used as biomarkers. After gene expression analysis, we could detect changes in the level of several genes, even at early time of exposition (6h), while classical biomarkers did not show modification compared with control. Thus, after verification, some genes could be selected to develop more specific and sensitive biomarkers for biomonitoring pesticide impact. Información suministrada por el agente en SIGEVA

Palabras Clave

RNA-SeqRhinella ArenarumAzinphos methylBiomarkers