Producción CyT
Congreso
Autoría
MARTIN, AYELEN
;
Clement Florencia
;
Venara Marcela
;
Maglio Silvana
;
García Lombardi Mercedes
;
Bergadá Ignacio
;
Pennisi Patricia A
Fecha
2016
Editorial y Lugar de Edición
Karger
Resumen
Información suministrada por el agente en
SIGEVA
Background: CNS tumors are the most frequent solid tumors in pediatric population. The IGF system of ligands and receptors are known to play an important role in both normal and neoplastic growth. Recently, quantitation of components of this system have been reported in CNS tumors from adult population, but information from pediatric patients is scarce.Objective: To characterize the expression of IGF-1, IGF-1R, IGF-2 and IR in CNS tumors from pediatric patients.Patients and Methods: We performe...
Background: CNS tumors are the most frequent solid tumors in pediatric population. The IGF system of ligands and receptors are known to play an important role in both normal and neoplastic growth. Recently, quantitation of components of this system have been reported in CNS tumors from adult population, but information from pediatric patients is scarce.Objective: To characterize the expression of IGF-1, IGF-1R, IGF-2 and IR in CNS tumors from pediatric patients.Patients and Methods: We performed a prospective study (6/2012-6/2016) of pediatric patients with CNS tumors without previous medical treatment that underwent surgery in our Hospital. Tissues were collected at the time of surgery. IGF-1, IGF-1R, IGF-2 and IR expression was measured by qRTPCR using extracted RNA. Specimens were classified according to localization or histology type and grade based on WHO2007 classification. Mann-Whitney, Kruskal-Wallis followed by Dunn´s Testwere used for comparisons.Results: We included 89 patients (50 males/39 females), median aged 8.9yrs, (range 0.9-18.6). The most common subgroups of CNS tumors were gliomas (n:36); ependymomas (n:16); medulloblastomas (n:11). Levels of IGF-1, IGF-1R, IGF-2 and IR mRNA were detectable in all specimens. Changes in IGF-2 mRNA levels among tumors were significantly different compared to the changes observed in the other genes analyzed (IGF-2:47(12-82) vs IGF-1:1(0.7-1.4); IGF-1R:0.8(0.5-1.0); IR:0.9(0.7-1.1),mean(CI) fold change, p<0.0001). In gliomas IGF-2 mRNA was lower while IGF-1 expression was higher in high grade compared to low grade tumors (p<0.05). IGF-IR and IR mRNAs were similar. Expression of IGF-2 and IR were lower in grade 2 and 3 ependymomas compared to grade 1, while IGF-1 and IGF-1R were similar among grades. When analyzed according to localization (supra/infratentorial, spinal cord) we found that IGF-1R expression was low in supratentorial ependymomas (p<0.001). Finally, we found no differences in IGF-1, IGF-1R, IGF-2 and IR expression levels between classic and anaplastic medulloblastomas.Conclusions: IGF-2 is highly variable in pediatric CNS tumors compared to other components of the IGF system. In gliomas and ependymomas IGF-2 was higher in lower grade tumors suggesting a role for IGF-2 in their biological behaviour. Further studies including patients´ follow up are necessary to confirm these results.
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Palabras Clave
IGF-1RGLIOMASCNSIGF-2