Producción CyT
Artículo
Autoría
OCAMPO, JOSEFINA
;
Feng Cui
;
Victor B. Zurkin
;
Clark D J
Fecha
2016
Editorial y Lugar de Edición
Landes Bioscience
Revista
nucleus,
vol. 7
(pp. 382-387)
- ISSN 1949-1034
Landes Bioscience
Landes Bioscience
ISSN
1949-1034
Resumen
Información suministrada por el agente en
SIGEVA
Eukaryotic DNA is packaged into regularly spaced nucleosomes, resembling beads on a string. Each bead contains »147 bp wrapped around a core histone octamer. Linker histone (H1) binds to the linker DNA to drive chromatin folding. Micrococcal nuclease (MNase) digestion studies reveal 2 mono-nucleosomal intermediates: the core particle (»147 bp) and the chromatosome (»160 bp; a core particle with additional DNA protected by H1). We have recently developed an improved method for ...
Eukaryotic DNA is packaged into regularly spaced nucleosomes, resembling beads on a string. Each bead contains »147 bp wrapped around a core histone octamer. Linker histone (H1) binds to the linker DNA to drive chromatin folding. Micrococcal nuclease (MNase) digestion studies reveal 2 mono-nucleosomal intermediates: the core particle (»147 bp) and the chromatosome (»160 bp; a core particle with additional DNA protected by H1). We have recently developed an improved method for mapping nucleosomes, using exonuclease III to remove residual linker (MNase-Exo- seq).1 We discovered 2 new intermediate particles corresponding to core particles with »7 bp of linker protruding from one side (»154 bp) or both sides (»161 bp), which are formed in the absence of H1. We propose that these ?proto-chromatosomes? are stabilised by core histone-DNA contacts in the linker, »7 bp from the nucleosome boundaries. These contacts may determine the topography of the H1 binding site.
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Palabras Clave
LINKER HISTONENUCLEOSOMECHROMATOSOMENUCLEOSOME MAPINGCHROMATIN FOLDINGMNASE-SEQMNASE-EXO-SEQPROTO-CHROMATOSOME