Producción CyT
1st LASID-SAI-FAIC Meeting. Book of Abstracts - VIP PROMOTES APOPTOTIC CELL PHAGOCYTOSIS BY MONOCYTES/MACROPHAGES THROUGH αvß3 INTEGRIN-THROMBOSPONDIN PORTAL FORMATION AND ENHANCED TROPHOBLAST MIGRATION IN A MODEL OF MATERNAL-PLACENTAL INTERACTION
Congreso
Autoría:
Daniel Paparini ; VOTA, DAIANA MARINA ; Esteban Grasso ; Vanesa Hauk ; Guillermina Calo ; Rosanna Ramhorst ; Claudia Pérez LeirósFecha:
2015Editorial y Lugar de Edición:
SAIResumen *
Background. Homeostasis maintenance at the early maternal-fetal interface requires active tissue remodelling processes and the clearance of apoptotic cells by macrophages in an immunosuppressant microenvironment. Vasoactive intestinal peptide (VIP) is a pleiotropic polypeptide synthesized by trophoblast cells that induces an anti-inflammatory profile in monocytes and macrophages (Mo/Ma). Here we explored VIP effect on trophoblast-monocyte interaction with special focus on Mo phagocytosis of apoptotic cells and trophoblast cell wound healing. Methods. Mo were purified by Percoll from PBMC of healthy volunteers and cultured in the presence/absence of VIP (1-100nM) or with conditioned media (CM) from first trimester human cytotrophoblast cell line (Swan-71) (Tb) treated or not with VIP (CM-VIP). Apoptosis was induced in Tb with camptothecin. CFSE-labelled apoptotic cell phagocytosis by CD14+ cells and αvß3 expression was assessed by flow cytometry and confocal microscopy. Thrombospondin1 expression was determined by RT-qPCR. Wound healing assays were run in trophoblast monolayers.Results. CM from Tb cells enhanced phagocytosis of apoptotic cells and induced αvß3 integrin re-localization and expression on Mo/Ma vs. DMEM (frecuency % X±SE: 77.1±2.0 vs. 49.6±1.5; P<0.05). CM-VIP further induced its expression (84.2±1.8). In parallel, thrombospondin1 expression was increased in VIP-treated Tb cells whereas an anti-thrombospondin1 mAb inhibited phagocytosis. Media from VIP-treated Mo/Ma were more efficient to promote Tb migration in wound healing assays than untreated cell media (P<0.05). Conclusions. These results are in line with a role of macrophages and trophoblast cells primed with VIP in the silent clearance of apoptotic cells and tissue remodelling processes required at early pregnancy. Información suministrada por el agente en SIGEVAPalabras Clave
VIPMONOCYTESPHAGOCYTOSISvß3 INTEGRIN