Producción CyT
Biocell 35(2):A132 - Expression of bone morphogenetic proteins BMP2, BMP4, BMP5 and BMP7 in the ampullary and isthmic regions of bovine oviduct during the oestrous cycle
Congreso
Fecha:
2011Editorial y Lugar de Edición:
INCAISSN:
0327-9545Resumen *
Bone morphogenetic proteins (BMPs) act as multifunctional regulators of embryonic development and play a crucial roles in mammalian reproduction. Our previous studies showed that BMP family members, receptors and their signaling pathway components are present in the oviduct, however, their functions are still unknown. In this study, we analyzed the expression of these factors during the oestrous cycle in the ampullary and isthmic regions of the bovine oviduct, considering that they play different functions during the reproductive process. The expression of BMP2, BMP4, BMP5 and BMP7 mRNA was studied by semiquantitative RT-PCR in epithelial cells obtained from the ampulla and isthmus at proestrus, metestrus and diestrus. -actin mRNA was used as internal reference gene in order to define the relative expression levels of each mRNA studied. The results showed the expression of BMP2, BMP4 and BMP7 mRNA in the region of ampulla and isthmus during the oestrous cycle. However, the expression of BMP5 mRNA was specific to epithelial cells from the isthmic region, suggesting certain function for this factor in the processes that occur in the isthmus. Información suministrada por el agente en SIGEVAPalabras Clave
BMPISTHMUSAMPULLABOVINE OVIDUCT