Producción CyT
Artículo
Autoría
WILKOWSKY, SILVINA ELIZABETH
;
Farber M
;
Echaide I
;
Torioni de Echaide, S
;
Zamorano P
;
Dominguez M
;
SUAREZ, C
;
FLORIN CHRISTENSEN, M
Fecha
2003
Editorial y Lugar de Edición
Elsevier
Revista
MOLECULAR AND BIOCHEMICAL PARASITOLOGY,
vol. 127
(pp. 133-141)
Elsevier
Resumen
Información suministrada por el agente en
SIGEVA
The search for vaccine candidates against bovine babesiosis caused by Babesia bovis is greatly focused on the identification of merozoite surface-exposed antigens that are widely conserved, functionally relevant and immunodominant in cattle protected against B. bovis infections. We have recently identified msa-2c, a member of the B. bovis VMSA gene family, which in contrast to other members, appears to be highly conserved among geographically distant B. bovis strains. In this study, we further ...
The search for vaccine candidates against bovine babesiosis caused by Babesia bovis is greatly focused on the identification of merozoite surface-exposed antigens that are widely conserved, functionally relevant and immunodominant in cattle protected against B. bovis infections. We have recently identified msa-2c, a member of the B. bovis VMSA gene family, which in contrast to other members, appears to be highly conserved among geographically distant B. bovis strains. In this study, we further investigated the potential of the msa-2c gene product as diagnostic and vaccine candidate for bovine babesiosis. RT-PCR and immunoblot studies demonstrated that MSA-2c is transcribed and translated in merozoites of the Argentine R1A strain. Anti recombinant MSA-2c (rMSA-2c) antibodies localized the protein on the parasite cell surface of R1A merozoites in immunofluorescence studies, and reacted in immunoblots with a single protein of approximately 30 kD in B. bovis merozoite extracts from both R1A and Australian “S” strains. Sera from cattle either naturally or experimentally infected with Argentine strains of B. bovis specifically recognized rMSA-2c in immunoblots, indicating that B-cell epitopes in rMSA-2c are conserved among field strains of B. bovis and are highly immunogenic, suggesting that MSA-2c may be a useful diagnostic tool for the detection of bovine babesiosis by B. bovis. Experimental vaccination of 5 bovines, with rMSA-2c resulted in elicitation of high specific anti-rMSA-2c IgG titers, with similar amounts of IgG1 and IgG2 produced. Importantly, bovine anti-rMSA-2c antibodies were able to neutralize in vitro bovine erythrocyte invasion by R1A merozoites suggesting a significant functional role for MSA-2c. Taken together these results postulate MSA-2c as a candidate for the development of novel tools for improved control of bovine babesiosis.
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