Producción CyT

Effects of extracellular nucleotides and their hydrolysis products on volume regulatory decrease of hepatocytes from trout (Oncorhynchus mykiss).

Artículo

Autoría:

DIEGO E, PAFUNDO ; MUT, Paula ; PERÉZ RECALDE, MERCEDES ; GONZALEZ LEBRERO, RODOLFO MARTIN ; FACHINO, VICTORIA ; KRUMSCHNABEL, G ; PABLO JOSÉ, SCHWARZBAUM

Fecha:

2004

Editorial y Lugar de Edición:

AMER PHYSIOLOGICAL SOC

Revista:

AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY, INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, vol. 287 (pp. 1-1) AMER PHYSIOLOGICAL SOC

Resumen *

In trout hepatocytes, hypotonic swelling is followed by a compensatory shrinkage called regulatory volume decrease (RVD). It has been postulated that extracellular ATP and other nucleotides may interact with type 2 receptors (P2) to modulate this response. In addition, specific ectoenzymes hydrolyze ATP sequentially down to adenosine, which may bind to type 1 receptors (P1) and also influence RVD. Accordingly, in this study, we assessed the role of extracellular nucleoside 5Ј-tri- and diphosphates and of adenosine on RVD of trout hepatocytes. The extent of RVD after 40 min of maximum swelling was denoted as RVD40, whereas the initial rate of RVD was called vRVD. In the presence of hypotonic medium (60% of isotonic), hepatocytes swelled 1.6 times followed by vRVD of 1.7 minϪ1 and RVD40 of 60.2%. ATP, UTP, UDP, or ATP␥S (P2 agonists; 5 ␮M) increased vRVD 1.5?2 times, whereas no changes were observed in the values of RVD40. Addition of 100 ␮M suramin or cibacron blue (P2 antagonists) to the hypotonic medium produced no effect on vRVD but a 53?58% inhibition of RVD40. Incubation of hepatocytes in the presence of either 5 ␮M [␥-32P]ATP or [␣-32P]ATP induced the extracellular release of [␥-32P]Pi (0.21 nmol 1⁄7 10Ϫ6 cellsϪ1 1⁄7 minϪ1) and [␣-32P]Pi (ϳ8 ϫ 10Ϫ3 nmol 1⁄7 10Ϫ6 cellsϪ1 1⁄7 minϪ1), suggesting the presence of ectoenzymes capable of fully dephosphorylating ATP. Concerning the effect of P1 activation on RVD, 5 ␮M adenosine, both in the presence and absence of 100 ␮M S-(4-nitrobenzil)-6-tioinosine (a blocker of adenosine uptake), decreased RVD40 by 37? 44%, whereas 8-phenyl theophylline, a P1 antagonist, increased RVD40 by 15%. Overall, results indicate that ATP, UTP, and UDP, acting via P2, are important factors promoting RVD of trout hepatocytes, whereas adenosine binding to P1 inhibits this process. Información suministrada por el agente en SIGEVA

Palabras Clave

nucleotides effectshepatocytesvolume regulation