Biocell - Isolation and Identification of Pyrrolizidine Alkaloids of Senecio rudbeckiaefolius MEYEN & WALPERS (ASTERACEAE)

Congreso

Resumen *

Senecio rudbeckiaefolius is a shrub that grows in the mountains of southern Perú, Solivia and northwestern Argentina. S. rudbeckiaefolius is considered toxic for livestock but is used in Peruvian traditional medicine for its diuretic, healing, anti-rheu-matic, antiinfective and menstrual period regulatory effects. The aim of this work was to identify the pirrolizidine alkaloids of its aerial parts. The plant material was collected in Tafi del Valle, Tucumán prov-ince. Aerial parts were extracted with methanol in a Soxhlet equip-ment and the solvent evaporated in rotavap. The residue was acidi-fied (H2S04, IN) and extracted with ethyl acétate. Zinc dust was added to the aqueous phase, the mixture was magnetically stirred for 24 hours, then alkalized to pH 10-11 and extracted with chloro-form. The chloroform extract was tested by TLC (detection with Dragendorff reagent), analyzed by gas chromatography coupled to mass spectrometry and ´H- and I3C-NMR. Two major alkaloids were isolated and identifíed: integerrimine and senecionine (ratio 8:1) with a yield of 1.14 mg and 0.14 mg per gram of dry plant material respectively. Two very minor additional alkaloids which are still under study were detected in the chloroform extract. Información suministrada por el agente en SIGEVA