Biocell - Transcriptional regulation of the fatty acid biosynthetic genes in Streptomyces coelicolor.

Congreso

Resumen *

Fatty acid biosynthesis is a vital facet of bacterial physiology and is carried out by a series of enzymatic steps, each encoded by a different gene known as the type II fatty acid synthase (FAS). In Streptomyces coelicolor the essential FAS genes are clustered at a single location within its genome (fab operon). Bioinformatics analysis of the fab genes locus revealed the presence of an open reading frame located upstream of fabD, named sco2386, which has homology with transcriptional regulators of polyketides biosynthesis in Streptomyces ssp. Sco2386 is highly conserved among several actinomycetes like Mycobacterium, Nocardia, Frankia, etc. In this work we investigated the functional role of Sco2386 in the transcriptional regulation of the fab operon and the physiological and morphological consequences of its mutation in S. coelicolor. For this, we constructed a sco2386 disruption mutant strain; evaluated its ability to growth under different carbon source and analyzed its fatty acid and TAG composition in different growth conditions. We also examined by reporter fusions the fab transcriptional profile in the sco2386 mutant as well as in the wild type strain. Finally we studied Sco2386-fabD promoter interactions by electrophoresis shift mobility assays. All the results obtained strongly suggest that Sco2386 mediates the transcriptional regulation of fab operon. Información suministrada por el agente en SIGEVA