Producción CyT
Artículo
Autoría
Fecha
2025
Editorial y Lugar de Edición
BJMAS ERCTD
Revista
British Journal of Multidisciplinary and advances Studies
- ISSN 2517-276X
BJMAS ERCTD
BJMAS ERCTD
ISSN
2517-276X
Resumen
Información suministrada por el agente en
SIGEVA
A B S T R A C TTomato has significant popularity due to its valuable nutrients content and bioactive components that benefit human health. This study aimed to investigate the antagonistic potential of Leuconostoc mesenteroides ssp. mesenteroides Tsc, a novel autochthonous microbial growth-inhibiting strain, against clinical isolates of Listeria monocytogenes and Salmonella Montevideo in a sterile tomato puree (TP, pH 4.1 +/- 0.2) incubated at 4 or 30 ºC for 30 days. At the same time, the e...
A B S T R A C TTomato has significant popularity due to its valuable nutrients content and bioactive components that benefit human health. This study aimed to investigate the antagonistic potential of Leuconostoc mesenteroides ssp. mesenteroides Tsc, a novel autochthonous microbial growth-inhibiting strain, against clinical isolates of Listeria monocytogenes and Salmonella Montevideo in a sterile tomato puree (TP, pH 4.1 +/- 0.2) incubated at 4 or 30 ºC for 30 days. At the same time, the effect of different inoculum sizes (4.0 or 6.0 log cfu/mL), and the changes in the content of ascorbic acid (nutritional quality ¨marker¨), organic acids and sensory profile were evaluated. Uninoculated or alone inoculated TPs were used as controls. Leuc. mesenteroides Tsc grew for all conditions studied, although slower at refrigeration temperature. By contrast, L. monocytogenes did not grow in TP under any assayed condition. However, its initial populations declined faster to below the detection limit (DL) when were co-inoculated with Leuc. mesenteroides Tsc relative to Listeria alone (on days 1 or 3 and 4 or 7 for low and high inoculum levels respectively) at 30 °C, while there were no significant differences in population survivals for all conditions tested at 4 ºC. Contrarily, Salmonella initial populations increased by ̴ 1.3 log CFU/ml within the first 24 h in TP when inoculated alone but not in the Leuc. mesenteroides Tsc presence. Pathogen populations were undetected on days 16 and 26 or 4 and 8 for low and high inoculation levels without or with Leuc. mesenteroides, respectively. Initial pH of TP inoculated with the Tsc strain regardless of the pathogen presence decreased from 4.10 to ~ 3.70 and significant lactic and acetic acids amounts were produced. However, it remained relatively unchanged throughout storage period when TP was inoculated with each test pathogen alone. Initial ascorbic acid concentration significantly decreased at the end of S. Montevideo grow in TP inoculated at 106 CFU/g at 30 ºC, whereas it maintained at high levels in fermented TP with strain Tsc. Thus, results obtained on growth/survival of two strains of human pathogens in TP highlight the potential of Leuc. mesenteroides Tsc as biocontrol agent to help to improve the safety of tomato products maintaining quality attributes, even though for L. monocytogenes its effectiveness was significantly influenced by the incubation temperature.Keywords. Salmonella, Listeria monocytogenes, Lactic acid fermentation, Tomato puree, Quality
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Palabras Clave
Lactic acid fermentationSalmonellaTomato puree qualityListeria monocytogenes