Producción CyT
Congreso
Autoría
Brenda Sede Lucena
;
ORDOÑEZ, OMAR FEDERICO
;
Tuttolomondo, M Victoria
;
Dallagnol, Andrea M
;
Mozzi, Fernanda
;
Pescuma, Micaela
Fecha
2025
Editorial y Lugar de Edición
SAMIGE
Resumen
Información suministrada por el agente en
SIGEVA
Calafate (Berberis microphylla) has high phenolic compounds (PC)concentration and has been used in traditional medicine to treat infectious diseases.Recently, we demonstrated that some lacticacid bacteria could grow in calafate fruit juice and modify their PCconcentration and antioxidant capacity. The aim of this study was to determinewhether the PC extracts of fermented calafate juices had antioxidant capacityand/or were capable of inhibiting the growth of human pathogenicmicroorganisms. The c...
Calafate (Berberis microphylla) has high phenolic compounds (PC)concentration and has been used in traditional medicine to treat infectious diseases.Recently, we demonstrated that some lacticacid bacteria could grow in calafate fruit juice and modify their PCconcentration and antioxidant capacity. The aim of this study was to determinewhether the PC extracts of fermented calafate juices had antioxidant capacityand/or were capable of inhibiting the growth of human pathogenicmicroorganisms. The calafate juices were fermented by Lacticaseibacillus casei B4and Fructobacillus fructosus B7 or by a co-culture of both strains at 30°C for 24 h. The juices were freeze-dried and two different extracts wereprepared, T (methanol: formic acid, 97:3, v/v), and A (methanol: 10% aceticacid, 85:15, v/v). From the T extract, flavonols (F) were separated fromanthocyanins (An) using a preparative C18 column. The solvents wereevaporated, and the samples were suspended in water. The PC concentration wasdetermined using the Folin-Ciocalteu method, and the antioxidant activity byABTS. The antimicrobial activity of the extracts on the strains Pseudomonasaeruginosa ATCC27853, Staphylococcus epidermidis ATCC12228 and Salmonellatyphimurium ATCC14028 was determined by plate diffusion (LB soft agar), andgrowth inhibition in LB broth was monitored at OD600nm using amicroplate reader. The PC concentration was higher in T extracts (1521 ± 397- 1838 ± 165 mgGAE/ml) than in A extracts (1093 ± 106 – 833 ± 28 mg GAE/ml). From the Textract, a fraction enriched in flavonols (792 ± 46 – 564 ± 28 mg GAE/ml) andanother one in anthocyanins (163 ± 10 - 136 ± 8 mg GAE/ml) were separated. Theantioxidant activity was higher in the T extract (7.0 ± 0.2%) and lower in theF extract (17 ± 0.2%) of the juice fermented by the co-culture compared to theextracts of the other samples. The An fraction showed antioxidant activity onlyfor the juice fermented by F. fructosus B7 (6.3 ± 0.9 µmol TE/mg GAE). The minimuminhibitory concentration (MIC) of T for P. aeruginosa was between 190and 95 mg GAE/ml, this value was lower than those of the juices fermented bythe strains separately. While for A, the MIC was higher for the extracts of thejuices fermented by B7 and the co-culture (52 mg GAE/ml). The MICs for St.epidermidis and S. typhimurium of T and A were lower than 47 and 26mg GAE/ml, respectively. The F fraction of the juice fermented by theco-culture was the only one that showed antimicrobial activity (MIC: 282 mgGAE/ml). When P. aeruginosa and St.epidermidis were grown in LB broth,the T and A extracts completely inhibited the strains' growth (100%), while theF fraction of the fermented juice by the co-culture showed an 80% inhibition.Moreover, this fraction inhibited S. typhimurium growth (23%) only after20 h of incubation. Our results show that lactic fermentation of calafate juicecould enhance the antioxidant and antimicrobial effects of calafate fruit extracts.
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Palabras Clave
calafate (Berberis microphylla)antioxidant and antimicrobial activitiesPhenolic compoundsfermented juice