Producción CyT
Artículo
Autoría
Biaggioni, Pablo Adrian
;
GONZALEZ, CAROLINA
Fecha
2026
Editorial y Lugar de Edición
WALTER DE GRUYTER & CO
Revista
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
WALTER DE GRUYTER & CO
Resumen
Información suministrada por el agente en
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Background: Accurate assessment of T-cell clonality is key for diagnosing mature T-cellneoplasms. TRBC1-based flow cytometry provides a rapid, robust, and cost-efficient approach.This systemaƟc review and meta-analysis assessed the diagnostic accuracy of TRBC1 flowcytometry (TRBC1-FC) for detecting T-cell clonality in mature T-cell neoplasms.Content: We systematically searched Scopus, PubMed (MEDLINE), and Google Scholar forarƟcles on TRBC1-FC diagnostic accuracy published up to July ...
Background: Accurate assessment of T-cell clonality is key for diagnosing mature T-cellneoplasms. TRBC1-based flow cytometry provides a rapid, robust, and cost-efficient approach.This systemaƟc review and meta-analysis assessed the diagnostic accuracy of TRBC1 flowcytometry (TRBC1-FC) for detecting T-cell clonality in mature T-cell neoplasms.Content: We systematically searched Scopus, PubMed (MEDLINE), and Google Scholar forarƟcles on TRBC1-FC diagnostic accuracy published up to July 1, 2025. Pooled sensitivity andspecificity were esƟmated, between-study heterogeneity was evaluated and small-studyeffects were examined.Summary: This meta-analysis included 10 studies. The pooled sensitivity was 97.6% (95% CI,95.1–99.4%) and specificity 90.7% (95% CI, 76.0–99.3%). The pooled LR+ was 10.9 (95% CI,4.1–28.9), LR- was 0.053 (95% CI, 0.025–0.12), and DOR 339 (95% CI, 64–1,788).The HSROC curve demonstrated an AUC of 0.974 (parƟal AUC = 0.970), confirming excellentglobal discriminatory capacity.Between-study heterogeneity was substantial (I² = 83.3%), mainly affecting specificity andDOR, while sensiƟvity remained highly consistent. No evidence of a threshold effect was found.Deeks’ test showed significant small-study effects (p < 0.001), and sensitivity analysesidentified one influenƟal study whose exclusion markedly reduced heterogeneity. Theseresults confirm the high diagnosƟc performance and robustness of TRBC1-FC for T-cell clonalityassessment.Outlook: TRBC1-FC demonstrates high sensitivity and low LR−, supporting its role as a rule-outtest. Variability in specificity, LR+ and DOR, mainly due to small-study effects, advises cautionfor rule-in use. Standardized protocols and cost-effecƟveness analyses are needed beforebroad clinical adoption.
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Palabras Clave
T-cell neoplasmsdiagnostic accuracyFlow CytometryTRBC1