Producción CyT
Congreso
Autoría
Dobladez, Valentina
;
Fernandez, María Celia
;
Pennisi, Patricia
;
MARTIN, AYELEN
Fecha
2025
Editorial y Lugar de Edición
Fundación Revista Medicina
Resumen
Información suministrada por el agente en
SIGEVA
Sex steroids play crucial roles in the growth and development of long bones. Glyphosate, a widely used herbicide, has controversial endocrine-disrupting effects. We demonstrated that exposure to low doses of glyphosate alters bone mineral density and quality in juvenile male rats, likely through modifications in matrix deposition during bone development. Objective: To analyze the possible role of sex steroids in modifying bone matrix deposition in cells exposed to low doses of glyphosate. Metho...
Sex steroids play crucial roles in the growth and development of long bones. Glyphosate, a widely used herbicide, has controversial endocrine-disrupting effects. We demonstrated that exposure to low doses of glyphosate alters bone mineral density and quality in juvenile male rats, likely through modifications in matrix deposition during bone development. Objective: To analyze the possible role of sex steroids in modifying bone matrix deposition in cells exposed to low doses of glyphosate. Methods: Human osteosarcoma cells (SaOS-2) were cultured under control conditions (C), and under estradiol (E2), testosterone (T), or dihydrotestosterone (DHT) treatment, with or without 100 ng/ml glyphosate (Gly). Cell viability and matrix deposition (Alizarin Red staining) under differentiation media were assessed. Results: Treatment with T increased cell viability, whereas DHT or E2 treatment had no significant effect (T:p=0.0022; DHT/E2:p=ns vs.C) on SaOS-2 cells. Glyphosate treatment reduced cell viability (Cvs.Gly:p=0.023). This reduction was also observed in co-culture with androgens (Tvs.T+Gly:p=0.004; DHTvs.DHT+Gly:p=0.05), but not with E2 (E2vs.E2+Gly:p=0.456). Under differentiation media, a decrease in mineralized matrix deposition was detected with T or E2 treatment, while DHT had no effect (T:p=0.004; E2:p=0.019; DHT:p=ns vs.C). Glyphosate treatment reduced matrix deposition (Cvs.Gly:p=0.006); this reduction persisted in co-culture with DHT (DHTvs. DHT+Gly:p=0.05), but not with T (Tvs.T+Gly:p=ns). In co-culture with E2, an increase in matrix deposition was detected (E2vs.E2+Gly:p=0.017), abrogating and surpassing the glyphosate effect. Conclusion: T -but not DHT- and E2 have differential effects on bone matrix deposition in the presence of glyphosate. Whether these effects are mediated by estrogenic pathways requires further investigation.
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Palabras Clave
Glyphosatebone developmentsex steroids