Producción CyT
Congreso
Autoría
Fecha
2023
Editorial y Lugar de Edición
LA-DOHAD
Resumen
Información suministrada por el agente en
SIGEVA
The aim of this study was to investigate whether exposure to the environmental pollutant 3-methylcholanthrene (3MC), considered to be an obesogenic agent, can enhance theadverse effects of overnutrition on reproductive development on male rat. To this end,prepubertal male rats fed standard or cafeteria diets were exposed to vehicle, 3MC (0.1mg/kg), alpha-naphthoflavone (αNF, 80 mg/kg) or a combination of them three times a weekfor 40 days. The study consisted of five experimental groups: ...
The aim of this study was to investigate whether exposure to the environmental pollutant 3-methylcholanthrene (3MC), considered to be an obesogenic agent, can enhance theadverse effects of overnutrition on reproductive development on male rat. To this end,prepubertal male rats fed standard or cafeteria diets were exposed to vehicle, 3MC (0.1mg/kg), alpha-naphthoflavone (αNF, 80 mg/kg) or a combination of them three times a weekfor 40 days. The study consisted of five experimental groups: controls (SDV), overweightrats (CDV), 3MC-exposed overweight rats (CD3MC), 3MC+αNF-exposed overweight rats(CD3MCαNF) and αNF-exposed overweight rats (CDαNF). At 61 days of age, blood, bonemarrow and spermatozoa were collected. Sperm count (million spermatozoa/ml), motility(percentage), morphology by Giemsa staining, presence of DNA fragmentation inlymphocytes by comet assay and metabolic profile were assessed. CICUAL of the Facultyof Medicine-UBA approved by resolution nº 768/2021. CDV rats showed a higher bodyweight (387±7 g; p<0.05) than SDV rats (349±10 g), and 3MC and αNF reduced thisincrease to SDV levels (p<0.05). Cholesterol, glucose and triglycerides circulating levelsshowed no differences between the groups. Compared to SDV rats (37±1), all CD rats hadlower sperm counts (CDV: 16±1; CD3MC: 13±2; CD3MCαNF: 15±3; CDαNF: 13±1;p<0.001). The CD groups also showed a decrease in motility percentage compared to SDVrats (15-50%). Furthermore, the CD3MC group showed a greater decrease in the numberof motile spermatozoa (34±2; p<0.001) and higher tail DNA content (2.7±0.7; p<0.05)compared to CDV rats (53±3; 0.9±0.2; respectively). Interestingly, αNF prevented this effect(52±2; 0.26±0.01; respectively). All groups exposed to αNF (43.0±0.2 days; p<0.05) showedearly puberty compared to SDV rats (44.2±0.2 days). These results indicate thatovernutrition and 3MC can lead to reproductive anomalies. Moreover, overnutrition mayincrease the susceptibility of developing organisms to environmental pollutants and impairreproductive function.
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Palabras Clave
OBESITYENVIRONMENTAL POLLUTANTMALE REPRODUCTION