Producción CyT
Congreso
Autoría
MARTIN, AYELEN
;
María Celia Fernandez
;
Mercedez Lombarte
;
Marina Cruz
;
Lucas Brun
;
Susana Zeni
;
Patricia Pennisi
Fecha
2024
Editorial y Lugar de Edición
Fundación Revista Medicina
Resumen
Información suministrada por el agente en
SIGEVA
Glyphosate is an herbicide used in agriculture for weed control. Thechronic effects of the herbicide at low doses have been controversial.Its potential to act as an endocrine disruptor affecting longitudinalgrowth during postnatal development is unknown. OBJECTIVE:To analyze the impact of exposure to low doses of glyphosate duringearly postnatal life on the development of long bones. METHODS:Female (8/16) and male (8/16) S-Dawley rats were treated daily fromdays 30 to 70 with 1 mg/kg/day of gly...
Glyphosate is an herbicide used in agriculture for weed control. Thechronic effects of the herbicide at low doses have been controversial.Its potential to act as an endocrine disruptor affecting longitudinalgrowth during postnatal development is unknown. OBJECTIVE:To analyze the impact of exposure to low doses of glyphosate duringearly postnatal life on the development of long bones. METHODS:Female (8/16) and male (8/16) S-Dawley rats were treated daily fromdays 30 to 70 with 1 mg/kg/day of glyphosate (EPA reference dose).On day 71 blood was collected. Bones (hind legs) were harvestedfor macroscopic,microscopic,biomechanic and RNAseq analysis.SaOS-2 cells were cultured in differentiation with/without glyphosateadded (10, 50, 100 ng/ml) for matrix deposition and expression profilingstudies on days 7,14, 21, 28 and 35. RESULTS: No differenceswere observed in body weight, renal or hepatic parameters, cortisol,E2, To or T3 between control and treated animals of both sexes. Tibias´ weight and lenght were similar in treated versus control animals.RNAseq studies showed no differentially expressed genes in femalesunder glyphosate treatment. In treated males, 54 genes weredifferentially expressed compared to controls (gene groups relatedto proteolysis, disassembly and organization of the extracellular matrix,collagen catabolic processing, and osteoblast differentiation).BMD was decreased (0.268±0.006 vs 0.253±0,002g/cm2p<0.03),while Stiffnes and Ultimate Load were higher (S:211±14vs285±23N;UL:78.3±1.5vs84.9±3.2N/mm, p<0.05) in treated males vs control.In vitro, SaOS2 cells exhibited significantly reduced mineralizedmatrix deposition (0.704±0.018 vs 0.301±0.169 mMd7; 3.12±0.2vs 2.27±0.10 mMd14,p<0.05 ANOVA) under glyphosate, with nodifferences in proliferation or apoptosis. CONCLUSION: Exposureto low doses of glyphosate alters the quality of developing bone injuvenile male rats, likely through modification of matrix deposition in developing bone
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Palabras Clave
GLYPHOSATEBONE DEVELOPMENT