Producción CyT
Artículo
Autoría
González-Espinosa, Francisco
;
Maurizi, Diego Marcelo
;
Pedersen, Dina
;
Oriani, Alejandra Soledad
;
Martinez, Celeste
;
Martínez Lozano, Jerson Andrés
;
Gutkind, Gabriel
;
Radice, Marcela
;
CEJAS, DANIELA
Fecha
2025
Editorial y Lugar de Edición
ASM
Revista
Microbiology Spectrum
ASM
Resumen
Información suministrada por el agente en
SIGEVA
Here, we characterized the first Klebsiella variicola isolates responsible foran outbreak in the intensive care units (ICUs) in a hospital in Argentina. Five isolatesrecovered from inpatients and sinks at the ICUs of one hospital in Bahia Blanca City wereincluded. Identification and antimicrobial susceptibility tests were performed using anautomated system (Phoenix). Besides, they were identified using two mass spectrometrysystems (Bruker Daltonics and BioMérieux) and a multiplex-PCR for...
Here, we characterized the first Klebsiella variicola isolates responsible foran outbreak in the intensive care units (ICUs) in a hospital in Argentina. Five isolatesrecovered from inpatients and sinks at the ICUs of one hospital in Bahia Blanca City wereincluded. Identification and antimicrobial susceptibility tests were performed using anautomated system (Phoenix). Besides, they were identified using two mass spectrometrysystems (Bruker Daltonics and BioMérieux) and a multiplex-PCR for Klebsiella pneumoniaecomplex identification. Whole-genome sequencing (WGS) was conducted to confirmbacterial identification and to analyze their resistome and mobilome. Additionally,clonal and phylogenetic relationships were investigated among local and worldwideisolates. Based on the Phoenix system, all isolates were identified as K. pneumoniaeand resistant to last-line antibiotics, consistent with a difficult-to-treat phenotype (DTR).All of them carried blaNDM-5. The isolates were identified as K. variicola by one of theMatrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) systems and by multiplex-PCR. WGS confirmed all isolates as K. variicola subsp.variicola belonging to ST3029, an infrequent lineage among the genomes analyzed.They displayed fewer than six SNPs, which corresponded to a unique clone, and thephylogenetic analysis exhibited a close relationship with isolates recovered from anenvironmental source in Brazil.
Ver más
Ver menos
Palabras Clave
Klebsiella variicolaoutbreakNDM-5ST3029