Producción CyT
Artículo
Autoría
Köster, Tino
;
Venhuizen, Peter
;
Lewinski, Martin
;
Petrillo, Ezequiel
;
Marquez, Yamile
;
Fuchs, Armin
;
Ray, Debashish
;
Nimeth, Barbara A.
;
Riegler, Stefan
;
Franzmeier, Sophie
;
RODRIGUEZ, FLORENCIA SOL
;
Aballay, Federico E.
;
Tognacca, Rocío S.
;
Zheng, Hong
;
Hughes, Timothy
;
Morris, Quaid
;
Barta, Andrea
;
Staiger, Dorothee
;
Kalyna, Maria
Fecha
2025
Editorial y Lugar de Edición
WILEY-BLACKWELL PUBLISHING, INC
Revista
NEW PHYTOLOGIST
WILEY-BLACKWELL PUBLISHING, INC
Resumen
Información suministrada por el agente en
SIGEVA
Alternative splicing is essential for plants, enabling a single gene to produce multiple transcript variants to boost functional diversity and fine-tune responses to environmental and developmental cues. Arabidopsis thaliana At-RS31, a plant-specific splicing factor in the Serine/Arginine-rich (SR) protein family, responds to light and the Target of Rapamycin (TOR) signalling pathway, yet its downstream targets and regulatory impact remain unknown.To identify At-RS31 targets, we applied individ...
Alternative splicing is essential for plants, enabling a single gene to produce multiple transcript variants to boost functional diversity and fine-tune responses to environmental and developmental cues. Arabidopsis thaliana At-RS31, a plant-specific splicing factor in the Serine/Arginine-rich (SR) protein family, responds to light and the Target of Rapamycin (TOR) signalling pathway, yet its downstream targets and regulatory impact remain unknown.To identify At-RS31 targets, we applied individual-nucleotide resolution crosslinking and immunoprecipitation (iCLIP) and RNAcompete assays. Transcriptomic analyses of At-RS31 mutant and overexpressing plants further revealed its effects on alternative splicing.iCLIP identified 4034 At-RS31 binding sites across 1421 genes, enriched in CU-rich and CAGA RNA motifs. Comparative iCLIP and RNAcompete data indicate that the arginine/serine (RS) domain of At-RS31 may influence its binding specificity in planta, underscoring the value of combining in vivo and in vitro approaches. Transcriptomic analysis showed that At-RS31 modulates diverse splicing events, particularly intron retention and exitron splicing, and influences other splicing modulators, acting as a hierarchical regulator.By regulating stress response genes and genes in both TOR and abscisic acid signalling pathways, At-RS31 may help integrate these signals, balancing plant growth with environmental adaptability through alternative splicing.
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Palabras Clave
ARABIDOPSIS THALIANASR proteinsALTERNATIVE SPLICINGRNAcompeteABAiCLIPbinding siteTOR kinase