Producción CyT
2025 MSA Annual Meeting - Fungal Futures: Cultivating Mycology and Mycologists - Ectomycorrhizal fungal gene expression linked to nitrogen decomposition rates and reduced bacterial nitrification
Congreso
Autoría:
Vietorisz, Corinne ; POLICELLI, NAHUEL ; Chikae Tatsumi ; Ko-Hsuan Chen ; Liao, Hui-Ling ; Bhatnagar, Jennifer M.Fecha:
2025Editorial y Lugar de Edición:
MSAResumen *
While soil microbes play central roles in nutrient cycling, it remains unclear how the activities ofdifferent fungal and bacterial functional groups, and associations between functional groups, arerelated to soil nutrient cycling rates. Ectomycorrhizal fungi (EMF), a dominant microbial groupin temperate forests, may impact nitrogen (N) cycling through their enzymatic and N-uptakeactivity, as well as through competition for N with other microbes. We tested the hypothesis thatmicrobial communities dominated by EMF will have higher nitrogen (N)-decomposition activity,with a corresponding increase in net ammonification rates, and decreases in bacterial nitrificationactivity and net nitrification rates. At six sites across Massachusetts, USA that varied in foreststand composition, we assessed fungal and bacterial community composition (via high-throughput sequencing of 16S and ITS2 rDNA amplicons) and gene expression (throughsequencing both the poly(A) enriched and ribo-zero rRNA depleted metatranscriptome) and soilbiogeochemical cycling rates. In support of our hypothesis, as EMF relative abundance and EMFN-cycling gene expression increased, net ammonification increased, while net nitrification andthe relative abundance of copiotrophic bacteria, especially nitrifying bacteria, decreased. N-decomposition gene expression by copiotrophic bacteria also increased. These findings suggestcompetition between EMF and copiotrophic bacteria for ammonium, causing a metabolic shiftaway from nitrification, a reduction in soil nitrification rates, and an increase in soilammonification rates. Información suministrada por el agente en SIGEVAPalabras Clave
MYCORRHIZAL FUNGIDECOMPOSITIONSOILNITROGEN