Producción CyT
Chylomicron characteristics are associated with microsomal triglyceride transfer protein in a diet-induced dysbiosis animal model.
Articulo
Autoría:
Olano, Carolina ; Fariña Gregorio ; Wiszniewski Morena ; Medel Jimena ; Morales, Celina ; Friedman, Silvia ; Macri Vanesa ; BARCHUK, MAGALÍ ; Berg Gabriela ; Schreier Laura ; Zago, ValeriaFecha:
2025Editorial y Lugar de Edición:
Korean Society of Lipid and AtherosclerosisRevista:
Journal of Lipid and Atherosclerosis Korean Society of Lipid and AtherosclerosisResumen *
Background and aims: Intestinal remnant chylomicrons (rCM) are involved in the cardiovascular residual risk and the atherogenic process. The microsomal triglyceride transfer protein (MTTP) catalyzes the assembly of lipids to apolipoprotein B48, generating CM. In dysbiosis state, their behavior could be altered impacting directly on CM characteristics. Our aim was to evaluate CM chemical composition, their association with intestinal MTTP and gut fat depots in a diet-induced dysbiosis animal model.Materials and methods: male Wistar rats were fed with standard diet (Control,n=10) or standard diet plus 40%fat+15% sucrose (High-Fat High-Sucrose Diet –HFSD-,n=10) for 14 weeks. Glucose, lipid-lipoprotein profile, free fatty acids, and lipopolysaccharides (LPS) were measured in sera and Firmicutes/Bacteroidetes (F/B) ratio from stool samples was determined by real time PCR quantification (RT-qPCR). Lipid components were measured in isolated CM (ultracentrifugation d<0.95g/ml). In intestinal tissue, MTTP was assessed by western blot, cell Intestinal fat content (CIF) by Folch extraction and histological study was performed; apoB mRNA levels and gut tight junction (TJ) proteins were quantified by RT-qPCR.Results: Compared to Control, HFSD showed higher LPS levels, triglycerides (TG), non HDL-C levels, TG/HDL-C, free fatty acids and F/B ratio. Lipid CM components from HFSD showed higher TG and phospholipids content. Lower levels of TJ proteins were verified in HFSD without differences in quantitative histological score. CIF was increased in HFSD. No differences were observed in apoB mRNA between groups. MTTP expression was higher in HFSD, and directly correlated with CM-TG and inversely correlated with CIF.Conclusion: our findings are consistent with a gut dysbiosis and insulin resistance development model, in which gut triglyceride content would constitute an important determinant of the secretion of triglyceride-rich CM, promoted by MTTP, with increased atherogenic potential. Información suministrada por el agente en SIGEVAPalabras Clave
ATHEROSCLEROSIS RISKDYSBIOSISINTESTINAL FATCHYLOMICRONS