Producción CyT
Libro de resúmenes del 14th Latin American and Caribbean Congress of Immunology, celebrado en Buenos Aires, Argentina, los días 4 al 8 de noviembre de 2024. - EXPLORING DP16(1)/YEY MOUSE MODEL TO UNDERSTAND THE EN- HANCED SUSCEPTIBILITY OF CHILDREN WITH TRISOMY 21 TO RESPIRATORY TRACT INFECTIONS: IMPACT OF A VI- RAL DOUBLE-STRANDED RNA MIMETIC ON LUNG IMMUNE CELL POPULATIONS
Congreso
Autoría:
Dutto, Jeremías ; Araya, Paula ; Boffelli Lucia ; Dhooge Sabrina ; Tosselo, Jimena ; Eliane Piaggio ; Núñez Nicolas Gonzalo ; Espinosa, Joaquín M. ; Maccioni, MarianaFecha:
2024Editorial y Lugar de Edición:
Sociedad Argentina de InmunologiaResumen *
Background: Children with Trisomy 21 (T21) are at a higher risk of contracting respiratory tract in- fections such as respiratory syncytial virus and more likely to require hospitalization with longer hospital stays and more probability of requiring ventilatory support and intensive care. Objec- tives: To evaluate the impact of poly IC, a dou- ble stranded RNA viral mimetic on the immune populations present in the lungs of Dp16(1)/Yey (DP16), a mouse model of T21. Methods: DP16 and control mice (n=12) were i.p. injected with poly IC and 15hs later, sacrificed. A 29-parameter spectral flow cytometry was used to characterize immune cells within spleen, lung, inguinal lymph nodes (iLN) and mesenteric lymph nodes (mLN). Results: At basal levels, most immune popula- tions present in lungs of DP16 mice showed a similar distribution than controls, except for a higher ratio of CD4+/CD8+ T cells and a signifi- cant reduction in natural killer cells (NK) (p<0.05). In contrast, upon poly IC stimulation the total number of leukocytes (CD45+ cells) diminishes in DP16 mice (p<0.05), and a huge remodeling of the immune compartment occurs: the frequency of B cells expressing activation markers such as CD69 augments (p<0.0001), indicating an hyper response in lungs of DP16 mice. Interestingly, among mature NK cells, the proportion of them expressing the chemokine receptor Cx3cr1 also significantly rises in DP16 mice (p<0.001). Of note, when overexpressed, Cx3cr1 chemokine Información suministrada por el agente en SIGEVAPalabras Clave
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