Producción CyT
Congreso
Autoría
Casajús, Victoria
;
BÁRCENA, ALEJANDRA
;
Martinez, Gustavo
;
Costa, Lorenza
Fecha
2025
Editorial y Lugar de Edición
Sociedad Argentina de Investigaciones en Bioquímicas y Biología Molecular (SAIB
Resumen
Información suministrada por el agente en
SIGEVA
Kale (Brassica oleracea var. sabellica) is a leafy vegetable whose consumption has increased significantly in recent years due to its high levels of health-promoting phytochemicals such as glucosinolates, vitamin C and polyphenols. Mature leaves are harvested for consumption. Therefore, the postharvest life of kale during storage and transport is characterized by the development of leaf senescence. The main symptom of leaf senescence is yellowing due to chlorophyll degradation. Chlorophyll degr...
Kale (Brassica oleracea var. sabellica) is a leafy vegetable whose consumption has increased significantly in recent years due to its high levels of health-promoting phytochemicals such as glucosinolates, vitamin C and polyphenols. Mature leaves are harvested for consumption. Therefore, the postharvest life of kale during storage and transport is characterized by the development of leaf senescence. The main symptom of leaf senescence is yellowing due to chlorophyll degradation. Chlorophyll degradation occurs via the pheophorbide-a-oxygenase pathway. Briefly, Chlorophyll b is converted to chlorophyll a by enzymes encoded by the NYC1, NOL and HCAR genes. The green pigment, Chlorophyll a, is then degraded by the sequential action of three enzymes encoded by the genes SGR1 (magnesium dechelatase), PPH (pheophytinase a) and PAO (pheophorbide oxygenase). PAO opens the ring causing the loss of the green color. An effective treatment for delaying postharvest yellowing in kale leaves is the irradiation during 1 h with low intensity pulses (25 μmol m-2 s-1) of red light every day. Red light has been reported to inhibit leaf senescence through phytochrome activation in Arabidopsis thaliana. The aim of this work is to analyze the effect of the low intensity red light treatment on the expression of genes involved in the postharvest chlorophyll degradation in kale leaves. For this purpose, kale leaves were divided into two groups: control (no light treatment) and red-light treatment. Kale leaves were separated into two groups: control (without light treatment) and red light treatment. After treatment, they were stored in dark. at 20 °C and 95% HR in darkness. Gene expression levels were determined by RTqPCR analysis. Putative BoNYC, BoNOL, BoSGR, BoPAO and BoPPH genes were identified based on the sequence similarity between Arabidopsis thaliana and Brassica oleracea using the data base available in NCBI. The expression of the five genes associated with chlorophyll degradation showed their induction in the darkness storage of kale leaves. The red light treatment inhibited the induction of the catabolic genes analyzed. BoNYC1, BoSGR and BoPAO showedthe strongest inhibitory effects. These results suggest that treatment with low intensity red light delays the yellowing of kale leaves through the regulation of the expression of genes encoding key enzymes of chlorophyll degradation pathway.
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Palabras Clave
TRANSCRIPTIONAL CONTROLKALE