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Book of Abstracts of the 75th Annual Meeting of the European Federation of Animal Science - The genomic homozygous landscape reveals selection sweeps in Argentinean Polo ponies
Congreso
Fecha:
2024Editorial y Lugar de Edición:
European Federation of Animal ScienceISSN:
979-12-210-6769-9Resumen *
The Caballo Polo Argentino is a landmark breed recognized worldwide as the most suitable for the practice of Polo. Despite its novelty (the breed was created 40 years ago), an intense selection process relied on the use of embryo transfer and cloning was performed. Here, we analyzed the genomic landscape of 385 polo ponies from 61 different breeders using runs of homozygosity (ROH)-based approach. Horses were genotyped using the Equine GGPV5 (Neogen, UK). The bioinformatics pipeline included an initial variant call and genotype curation using Genome Studio and Plink. ROH call was performed by a sliding-windows approach in DetectRUNS using the following parameters WindSize:30; minSNP:10; thr:0,05; maxGap:10^6; mLen:1000000bp, maxOpp:0 and max-Miss:1. ROH analysis and genomic annotation of ROH islands (ROHi) was performed using Bioconductor in R. We detected 42,727 ROH (111 per individual). 80% of the ROH were short (1-5Mb), but 70% of the individuals showed ROH ≥16.6Mb (associated with very recent inbreeding). On average, the genomic inbreeding coefficient based on ROH (FROH) was 14.3% showing a low correlation with pedigree-based inbreeding coefficient (~0.3, probably associated with weak pedigree records). It also shows a highly variable pattern at the chromosome level. 27% of the FROH was associated with recent inbreeding (probably by the heavy use of large-scale reproductive biotechnology programs). Four ROHi (located in 60% of the samples) were detected in ECA1, ECA7, and ECA17, including 25 genes. Some of them were previously associated with reproductive and metabolic functions in mammals. To our knowledge, this is the first large-scale genomic characterization of Polo Argentino. Información suministrada por el agente en SIGEVAPalabras Clave
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